GENOMICS AND BIOTECHNOLOGY OF PLANT-ASSOCIATED MICROORGANISMS (GeBioPAM)
Group leader: Juan Imperial Ródenas - Research Professor CSIC-INIA
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Personnel:
- Molinero Gallego, Cristina - PhD Student
- Rojas López-Menchero, Jorge - PhD Student
- Salinero Lanzarote, Álvaro - Technician
We study plant-associated microorganisms (bacteria and fungi). We use -omic tools to advance our knowledge on their diversity and evolution, as well as on their negative (pathogenic) and positive (role in plant nutrition, growth, development, and protection) effects on crops, both individually and as part of plant microbiomes.
The Group’s objectives are centred on
- Diversity and evolutionary studies of plant pathogenic (Xylella fastidiosa) plant symbiotic (Rhizobia), and plant-associated (endophytic fungi, free-living diazotrophs) microorganisms through microbiological and genomic approaches.
- Studies on the composition and role of plant microbiomes.
- Biotechnological exploitation of plant-associated microorganisms and of plant microbiomes to enhance plant nutrition, development, growth and health in crops.
We strive to attain these through long-standing collaborations
On Xylella fastidiosa with Dr. Blanca Landá’s group at IAS-CSIC
On endophytic fungi with Dr. Azucena González-Coloma’s group at ICA-CSIC
On diazotrophic Pseudomonads with Dr. Luis Rubio (CBGP) and with Prof. Jorge Lalucat (UIB)
On Rhizobial legume symbionts with Prof. Manuel González-Guerrero (CBGP).
Funding and Publications
See ORCID
Reyes Castillo, N., Díaz, C.E., Andres, M.F., Imperial, J., Valcárcel, F., González Coloma, A.A. 2024. Optimization of fungicidal and acaricidal metabolite production by endophytic fungus Aspergillus sp. SPH2. Bioresources and Bioprocessing 11, 28. DOI: 10.1186/s40643-024-00745-9
Navarro-Gómez, C., León-Mediavilla, J., Küpper, H., Rodríguez-Simón, M., Paganelli-López, A., Wen, J., Burén, S., Mysore, K.S., Bokhari, S.N.H., Imperial, J., Escudero, V., González-Guerrero, M. 2023. Nodule-specific Cu+-chaperone NCC1 is required for symbiotic nitrogen fixation in Medicago truncatula root nodules. New Phytologist. DOI: 10.1111/nph.19360
Barahona, E., Isidro, E.S., Sierra-Heras, L., Álvarez-Melcón, I., Jiménez-Vicente, E., Buesa, J.M., Imperial, J., Rubio, L.M. 2022. A directed genome evolution method to enhance hydrogen production in Rhodobacter capsulatus. Frontiers in Microbiology 13. DOI: 10.3389/fmicb.2022.991123
Tighilt, L., Boulila, F., De Sousa, B.F.S., Giraud, E., Ruiz-Argüeso, T., Palacios, J.M., Imperial, J., Rey, L. 2021. The Bradyrhizobium Sp. LmicA16 Type VI Secretion System Is Required for Efficient Nodulation of Lupinus Spp.. Microbial Ecology 1–12. DOI: 10.1007/s00248-021-01892-8
Msaddak, A., Rey, L., Imperial, J., Palacios, J.M., Mars, M., Pueyo, J.J. 2021. Phylogenetic Analyses of Rhizobia Isolated from Nodules of Lupinus angustifolius in Northern Tunisia Reveal Devosia sp. as a New Microsymbiont of Lupin Species. Agronomy 11, 1510. DOI: 10.3390/agronomy11081510
Castro-Rodríguez, R., Escudero, V., Reguera, M., Gil-Díez, P., Quintana, J., Prieto, R.I., Kumar, R.K., Brear, E., Grillet, L., Wen, J., Mysore, K.S., Walker, E.L., Smith, P.M.C., Imperial, J., González-Guerrero, M. 2021. Medicago truncatula Yellow Stripe-Like7 encodes a peptide transporter participating in symbiotic nitrogen fixation. Plant, Cell & Environment. DOI: 10.1111/pce.14059
Senovilla, M., Abreu, I., Escudero, V., Cano, C., Bago, A., Imperial, J., González-Guerrero, M. 2020. MtCOPT2 is a Cu+ transporter specifically expressed in Medicago truncatula mycorrhizal roots. Mycorrhiza. DOI: 10.1007/s00572-020-00987-3
Castro-Rodríguez, R., Abreu, I., Reguera, M., Novoa-Aponte, L., Mijovilovich, A., Escudero, V., Jiménez-Pastor, F.J., Abadía, J., Wen, J., Mysore, K.S., Álvarez-Fernández, A., Küpper, H., Imperial, J., González-Guerrero, M. 2020. Medicago truncatula Yellow Stripe1-Like3 gene is involved in vascular delivery of transition metals to root nodules. Journal of Experimental Botany. DOI: 10.1093/jxb/eraa390
Escudero, V., Abreu, I., Tejada‐Jiménez, M., Rosa‐Núñez, E., Quintana, J., Prieto, R.I., Larue, C., Wen, J., Villanova, J., Mysore, K.S., Argüello, J.M., Castillo‐Michel, H., Imperial, J., González‐Guerrero, M. 2020. Medicago truncatula Ferroportin2 mediates iron import into nodule symbiosomes. New Phytologist. DOI: 10.1111/nph.16642
Velázquez-Lam, E., Imperial, J., Ponz, F. 2020. Polyphenol-Functionalized Plant Viral-Derived Nanoparticles Exhibit Strong Antimicrobial and Antibiofilm Formation Activities. ACS Applied Bio Materials. DOI: 10.1021/acsabm.9b01161
Escudero, V., Abreu, I., del Sastre, E., Tejada-Jiménez, M., Larue, C., Novoa-Aponte, L., Castillo-González, J., Wen, J., Mysore, K.S., Abadía, J., Argüello, J.M., Castillo-Michel, H., Álvarez-Fernández, A., Imperial, J., González-Guerrero, M. 2020. Nicotianamine Synthase 2 Is Required for Symbiotic Nitrogen Fixation in Medicago truncatula Nodules. Frontiers in Plant Science 10, 1780. DOI: 10.3389/fpls.2019.01780
Soenens, A., Imperial, J. 2019. Biocontrol capabilities of the genus Serratia. Phytochemistry Reviews. DOI: 10.1007/s11101-019-09657-5
Torres, A.R., Brito, B., Imperial, J., Palacios, J.M., Ciampitti, I.A., Ruiz-Argüeso, T., Hungria, M. 2020. Hydrogen-uptake genes improve symbiotic efficiency in common beans (Phaseolus vulgaris L.). Antonie van Leeuwenhoek. DOI: 10.1007/s10482-019-01381-6
Msaddak, A., Rejili, M., Durán, D., Mars, M., Palacios, J.M., Ruiz-Argüeso, T., Rey, L., Imperial, J. 2019. Microvirga tunisiensis sp. nov., a root nodule symbiotic bacterium isolated from Lupinus micranthus and L. luteus grown in Northern Tunisia. Systematic and Applied Microbiology 126015. DOI: 10.1016/j.syapm.2019.126015
Soenens, A; Gomila, M; Imperial, J. 2018. "Neorhizobium tomejilense sp. nov., first non-symbiotic Neorhizobium species isolated from a dryland agricultural soil in southern Spain". Systematic and Applied Microbiology. DOI: 10.1016/j.syapm.2018.09.001".
León-Mediavilla, J; Senovilla, M; Montiel, J; Gil-Díez, P; Saez, Á; Kryvoruchko, IS; Reguera, M; Udvardi, MK; Imperial, J; González-Guerrero, M. 2018. "MtMTP2-facilitated zinc transport into intracellular compartments is essential for nodule development in Medicago truncatula". Frontiers in Plant Science. DOI: 10.3389/fpls.2018.00990".
Gil-Díez, P; Tejada-Jiménez, M; León-Mediavilla, J; Wen, J; Mysore, KS; Imperial, J; González-Guerrero, M. "MtMOT1.2 is responsible for molybdate supply to Medicago truncatula nodules". Plant, Cell & Environment. DOI: 10.1111/pce.13388".
Soenens, A; Imperial, J. 2018. "Novel, non-symbiotic isolates of Neorhizobium from a dryland agricultural soil". PeerJ. DOI: 10.7717/peerj.4776".
Msaddak, A; Rejili, M; Durán, D; Rey, L; Palacios, JM; Imperial, J; Ruiz-Argüeso, T; Mars, M. 2018. "Definition of two new symbiovars, sv. lupini and sv. mediterranense, within the genera Bradyrhizobium and Phyllobacterium efficiently nodulating Lupinus micranthus in Tunisia". Systematic and Applied Microbiology. DOI: 10.1016/j.syapm.2018.04.004".
Ahnia, H; Bourebaba, Y; Durán, D; Boulila, F; Palacios, JM; Rey, L; Ruiz-Argüeso, T; Boulila, A; Imperial, J. 2018. "Bradyrhizobium algeriense sp. nov., a novel species isolated from effective nodules of Retama sphaerocarpa from Northeastern Algeria". Systematic and Applied Microbiology. DOI: 10.1016/j.syapm.2018.03.004".
Sánchez-Cañizares, C; Jorrín, B; Durán, D; Nadendla, S; Albareda, M; Rubio-Sanz, L; Lanza, M; González-Guerrero, M; Prieto, R; Brito, B; Giglio, M; Rey, L; Ruiz-Argüeso, T; Palacios, J; Imperial, J. 2018. "Genomic diversity in the endosymbiotic bacterium Rhizobium leguminosarum". Genes. DOI: 10.3390/genes9020060".
Senovilla, M; Castro-Rodríguez, R; Abreu, I; Escudero, V; Kryvoruchko, I; Udvardi, MK; Imperial, J; González-Guerrero, M. 2018. "Medicago truncatula copper transporter 1 (MtCOPT1) delivers copper for symbiotic nitrogen fixation". New Phytologist. DOI: 10.1111/nph.14992".
Armanhi, JSL; de Souza, RSC; Damasceno, NdB; de Araújo, LM; Imperial, J; Arruda, P. 2018. "A community-based culture collection for targeting novel plant growth-promoting bacteria from the sugarcane microbiome". Frontiers in Plant Science. DOI: 10.3389/fpls.2017.02191".
Durán, D; Imperial, J; Palacios, J; Ruiz-Argüeso, T; Göttfert, M; Zehner, S; Rey, L. 2017. "Characterization of a novel MIIA domain-containing protein (MdcE) in Bradyrhizobium spp". FEMS Microbiology Letters. DOI: 10.1093/femsle/fnx276".
Keller, J; Imperial, J; Ruiz-Argüeso, T; Privet, K; Lima, O; Michon-Coudouel, S; Biget, M; Salmon, A; Aïnouche, A; Cabello-Hurtado, F. 2017. "RNA sequencing and analysis of three Lupinus nodulomes provide new insights into specific host-symbiont relationships with compatible and incompatible Bradyrhizobium strains". Plant Science. DOI: 10.1016/j.plantsci.2017.10.015".
Tejada-Jiménez, M; Gil-Díez, P; León-Mediavilla, J; Wen, J; Mysore, KS; Imperial, J; González-Guerrero, M. 2017. "Medicago truncatula Molybdate Transporter type 1 (MtMOT1.3) is a plasma membrane molybdenum transporter required for nitrogenase activity in root nodules under molybdenum deficiency". New Phytologist. DOI: 10.1111/nph.14739".
Abreu, I; Saéz, Á; Castro-Rodríguez, R; Escudero, V; Rodríguez-Haas, B; Senovilla, M; Larue, C; Grolimund, D; Tejada-Jiménez, M; Imperial, J; González-Guerrero, M. 2017. "Medicago truncatula Zinc-Iron Permease6 provides zinc to rhizobia-infected nodule cells". Plant, Cell & Environment. DOI: 10.1111/pce.13035".
Msaddak, A; Rejili, M; Duran, D; Rey, L; Imperial, J; Palacios, J; Ruiz-Argueso, T; Mars, M. 2017. "Members of Microvirga and Bradyrhizobium genera are native endosymbiotic bacteria nodulating Lupinus luteus in Northern Tunisian soils". FEMS Microbiology Ecology. DOI: 10.1093/femsec/fix068".
Msaddak, A; Durán, D; Rejili, M; Mars, M; Ruiz-Argüeso, T; Imperial, J; Palacios, J; Rey, L. 2017. "Diverse bacteria affiliated with the genera Microvirga, Phyllobacterium and Bradyrhizobium nodulate Lupinus micranthus growing in soils of Northern Tunisia". Applied and Environmental Microbiology. DOI: 10.1128/aem.02820-16".
Armanhi, JSL; de Souza, RSC; de Araújo, LM; Okura, VK; Mieczkowski, P; Imperial, J; Arruda, P. 2016. "Multiplex amplicon sequencing for microbe identification in community-based culture collections". Scientific Reports. DOI: 10.1038/srep29543".
de Souza, RS; Okura, VK; Armanhi, JS; Jorrin, B; Lozano, N; da Silva, MJ; Gonzalez-Guerrero, M; de Araujo, LM; Verza, NC; Bagheri, HC; Imperial, J; Arruda, P. 2016. "Unlocking the bacterial and fungal communities assemblages of sugarcane microbiome". Scientific Reports. DOI: 10.1038/srep28774".
Bourebaba, Y; Durán, D; Boulila, F; Ahnia, H; Boulila, A; Temprano, F; Palacios, JM; Imperial, J; Ruiz-Argüeso, T; Rey, L. 2016. "Diversity of Bradyrhizobium strains nodulating Lupinus micranthus on both sides of the Western Mediterranean: Algeria and Spain". Systematic and Applied Microbiology. DOI: 10.1016/j.syapm.2016.04.006".
Tejada-Jiménez, M; Castro-Rodríguez, R; Kryvoruchko, I; Lucas, MM; Udvardi, M; Imperial, J; González-Guerrero, M. 2015. "MtNramp1 is required for iron uptake by rhizobia-infected Medicago truncatula nodule cells". Plant Physiology. DOI: 10.1104/pp.114.254672".
Albareda, M; Rodrigue, A; Brito, B; Ruiz-Argueso, T; Imperial, J; Mandrand-Berthelot, M-A; Palacios, J. 2015. "Rhizobium leguminosarum HupE is a highly-specific diffusion facilitator for nickel uptake". Metallomics. DOI: 10.1039/C4MT00298A".
Jorrin, B; Imperial, J. 2015. "Population genomics analysis of legume host preference for specific rhizobial genotypes in the Rhizobium leguminosarum bv. viciae symbioses". Molecular Plant-Microbe Interactions. DOI: 10.1094/MPMI-09-14-0296-FI".
Durán, D; Rey, L; Sánchez-Cañizares, C; Navarro, A; Imperial, J; Ruiz-Argüeso, T. 2013. "Genetic diversity of indigenous rhizobial symbionts of the Lupinus mariae-josephae endemism from alkaline-limed soils within its area of distribution in Eastern Spain". Systematic and Applied Microbiology. DOI: S0723-2020(12)00159-2 [pii] 10.1016/j.syapm.2012.10.008".
Ormeño-Orrillo, E; Servín-Garcidueñas, LE; Imperial, J; Rey, L; Ruiz-Argüeso, T; Martínez-Romero, E. 2013. "Phylogenetic evidence of the transfer of nodZ and nolL genes from Bradyrhizobium to other rhizobia". Molecular Phylogenetics and Evolution. DOI: S1055-7903(13)00099-7 [pii] 10.1016/j.ympev.2013.03.003".
Durán, D; Rey, L; Sánchez-Cañizares, C; Jorrín, B; Imperial, J; Ruiz-Argüeso, T. 2013. "Biodiversity of Slow-Growing Rhizobia: The Genus Bradyrhizobium", p. 21-46. In B. Rodelas and J. González (ed.), Beneficial Plant-microbial Interactions: Ecology and Applications. CRC Press.
Rodríguez-Haas, B; Finney, L; Vogt, S; González-Melendi, P; Imperial, J; González-Guerrero, M. 2013. "Iron distribution through the developmental stages of Medicago truncatula nodules". Metallomics. DOI: 10.1039/c3mt00060e".
Rubio-Sanz, L; Prieto, RI; Imperial, J; Palacios, JM; Brito, B. 2013. "Functional and expression analysis of the metal-inducible dmeRF system from Rhizobium leguminosarum bv. viciae". Applied and Environmental Microbiology. DOI: AEM.01954-13 [pii] 10.1128/AEM.01954-13".
Albareda, M.; Manyani, H.; Imperial, J.; Brito, B.; Ruiz-Argueso, T.; Bock, A.; Palacios, J.M. 2012. Dual role of HupF in the biosynthesis of [NiFe] hydrogenase in Rhizobium leguminosarum. BMC Microbiology 12:256.