Thanks to the PANGREEN project, the CBGP Green BioFactory for Health (GreenBIO4H) infrastructure has been reinforced for cheap, rapid and massive production in plants of new proteins/peptides and other biomolecules with potential applications to Biohealth use, such as antigens for diagnostic tests and therapies to fight pandemics. The available infrastructures have been improved to guarantee the scalability and optimize the performance of GreenBio4H, giving coverage to most of the R+D+i agents of the Community of Madrid. It will allow to lead the production of biomolecules of health interest in plants and responding to future pandemics and other health challenges.

The multidisciplinary project PANGREEN-CM is part of the Agreement between the Community of Madrid (Ministry of Education, Universities, Science and Spokesperson of Madrid Regional Government) and the Technical University of Madrid for the direct granting of financial support research on SARS -COV2 and the COVID 19 disease. This grant has been financed with REACT-EU resources of the European Regional Development Fund.

Acción/contrato financiada/o mediante el CONVENIO ENTRE LA COMUNIDAD DE MADRID (CONSEJERÍA DE EDUCACIÓN, UNIVERSIDADES, CIENCIA Y PORTAVOCÍA) Y LA UNIVERSIDAD POLITÉCNICA DE MADRID PARA LA CONCESIÓN DIRECTA DE UNA AYUDA DE 7.912.000,00 EUROS PARA FINANCIAR LA REALIZACIÓN DE ACTUACIONES EN MATERIA DE INVESTIGACIÓN SOBRE EL SARSCOV2 Y LA ENFERMEDAD COVID-19 FINANCIADO CON LOS RECURSOS REACT-UE DEL FONDO EUROPEO DE DESARROLLO REGIONAL.


 

It is located in Laboratory S51 (CBGP Metabolomics Platform) that is integrated in the Network of Laboratories and Infrastructures of Madrid Region.

Responsable: Araceli Díaz Perales - Professor
araceli.diaz@upm.es 910679170 910679129 ( Lab 151/S78)

 

HPLC is used to perform protein/peptide purification and to analyze proteins/peptides from complex samples, as well as their characterization as a function of their time of elution. Although its main application is focused on protein/peptide purification processes, HPLC is also used for the identification of compounds by analytical chromatography and comparison of elution profiles.

 

The main technical characteristics of HPLC equipment are:

  1. High pressure liquid chromatography system designed to obtain purified proteins from complex mixtures, allowing purification in the milligram range. Due to this versatility of the equipment (system with dynamic flow), it allows working up to, at least, the range of 7.5 ml/min at maximum pressures of at least 600 bar, allowing its application in the wide range of workflows that can be generated.
  2. Detection Unit is equipped with a variable wavelength detector, with the possibility of working at two simultaneous wavelengths. It also has a fluorescence detector, which after the derivatization of the compounds is capable of significantly increasing the sensitivity of the UV-VIS detector, for the purification of compounds that are poorly represented in the raw material.
  3. To guarantee the automation of the processes, HPLC has a fraction collector.



 

It is located in Laboratory S51 (CBGP Metabolomics Platform) that is integrated in the network of Laboratories and Infrastructures of Madrid Region.

Responsable: Antonio Molina Fernández - Professor
antonio.molina@upm.es 910679199 / 910679213 - 910679201 ( Lab 235)

 

It is used for the analysis of biological compounds (sugars, lipids and metabolites) from complex samples, as well as their characterization and biochemical identification.

 

The main technical characteristics are the following:

  1. Modular Ion chromatography system working in the entire pH range (0-14).
  2. Inert and metal-free high-pressure pumps to be able to work at extreme pH
  3. It allows working with all kinds of isocratic IC separations, with gradients of different mobile phases and with the capacity to carry out gradients of different concentrations with up to four different mobile phases (carbonate/bicarbonate, hydroxide...).
  4. It has a degasser for the mobile phase.
  5. Includes adjustable thermostat for column module.
  6. It allows working with columns with small particle size.
  7. Flexible flow rate for 2, 3, 4, 5 or 9 mm columns.
  8. Low production of residual elements and low consumption of reagents.



 

It is located in Laboratory S51 (CBGP Metabolomics Platform) that is integrated in the network of Laboratories and Infrastructures of Madrid Region.

Responsable: María Garrido Arandia - Assistant Professor
maria.garrido@upm.es 910679215 / 910679129 ( Lab 151/S78)

 

It is mainly used to carry out studies of biomolecular interactions between molecules (e.g. produced on the CBGP PANGREEN platform and purified on the HPLC and HPIC platforms), and receptor-ligan binding assays.

 



 

It is located in the CBGP greenhouses (Module 11). Integrated into the CBGP Green BioFactory for Health (GreenBIO4H) infrastructure. This GreenBIO4H agroinfiltration system is part of a new Laboratory that will be integrated into the Network of Laboratories and Infrastructures of Madrid Region.


Responsable: Fernando Ponz Ascaso - Research Professor CSIC-INIA
fponz@inia.csic.es 910679187 / 910679145 ( Lab 271 )


It is used to carry out experiments to produce proteins and other biomolecules of interest in plants and to semi-automatize the production process to scaling it up. The main application of the equipment is focused on the infiltration processes of plants of the Nicotiana benthamiana species with different constructs transformed in Agrobacterium tumefaciens.

The main technical characteristics of the equipment are the following:
  1. Designed to carry out automated agroinfiltration of Nicotiana benthamiana plants and other plant species by immersion in Agrobacterium tumefaciens solution, that upon vacuum application enhance the agroinfiltration of the bacterium in plant tissues.
  2. It has immersion baths for the plants that are compartmentalized, allowing efficiency in the use of reagents and plants.
  3. The system allows the visualization of the process to guarantee the correct execution of the different steps.



 

It is located in Laboratory 234/232 ntegrated into the Biofoundry of the SynBIO2 infrastructure of the CBGP, that will be integrated into Network of Laboratories and Infrastructures of the Madrid Region.

 

This robotic equipment (2 similar Units) is used for the manipulation of liquids and the automation of molecular biology and genetic engineering protocols that are necessary to standardize synthetic biology processes.
 
The main technical characteristics are the following:
  1. Designed to carry out the automation of molecular biology protocols such as dilutions, generation of libraries or preparation for techniques such as qPCR. The equipment can scale these protocols to work with 96-sample plates, up to 8-channel pipettors, 5% precision to 1µl.
  2. The equipment has the modules necessary to carry out said protocols: thermal cycler module, magnetic module and HEPA. These modules are included within the kit, so that the pipettors can manipulate the liquids between the modules and the 96-well plates.
  3. With two identical basal modules to have the greatest versatility of automation programming.
  4. Integrated software that allows the programming of protocols in a simple way. It has an API or programming interface to use known languages (such as Python) when implementing protocols.