Microtome Leica HistoCore NANOCUT R

1. Automatic or manual working mode.
2. Ergonomic and independent control panel.
3. Holders for paraffin-wax and resin embedded specimens.
4. Holders for steel and diamond knifes.
5. Minimum sectioning thickness 0,5 µm.
6. Integrated stereomicroscope.

Vibratome Leica VT1200 S

1. Thick sections from non-embedded specimens by blade (Guillete) vibration.
2. Automatic or manual working mode.
3. Ergonomic and independent control panel.
4. Integrated stereomicroscope.

Paraffin-wax dispenser Leica EG 1110

Hybridisation oven Amersham

• Fluorescence cubes:
  
  

• Objectives:
  
  

*DIC = differential interference contrast

Coupled to colour CMOS camera DC.20000i euromex (20 Mpixels).

• Objetives:
  
  

1. diascopic and episcopic illumination
2. plan-apochromatic objective 1.0x/0.125
3. zoom 0.8x – 8x
4. fluorescence cubes
   
   

Coupled to colour CCD camera Leica DFC 400C (5 Mpixels).

• Fluorescence cubes:
  
  

• Objectives:
  
  

Coupled to colour CCD camera Leica DFC 300FX (1,4 Mpixels).

1. episcopic illumination
2. objectives 0.5x y 1.0x
3. zoom 0.63x – 5.7x
4. fluorescence cubes

1. episcopic illumination
2. objective PLAN 1.0x
3. zoom 0.63x – 6x

Inverted microscope Leica DMI6000CS 

1. touchscreen STP6000

2. smart move (moves the stage in XY and Z) 

3. metal halide bulb EL6000 (120 W)

4. Fluorescence clubes:
   
   

• objectives:
  
  

*BF = bright field, DIC = differential interference contrast, FLUO = fluorescence

Confocal module

FOV scanner: 

1. speed up to 1.800 Hz (3.600 Hz in bidirectional mode)
2. up to 7 fps @ 512 x 512 px
3. image format up to 8.192 x 8.192 px

Lasers: 

1. CW (405 nm). 50 mW
2. Ar (458, 488 y 514 nm). 100 mW
3. DPSS (561 nm). 20 mW
4. He-Ne (633 nm). 10 mW

Detectors: 

1. 4 spectral for fluorescence and reflection (2 PMT and 2 HyD)
2. 1 (non-confocal) for transmitted light

LIAchroic beam splitter, which includes the dichroic mirrors: 

1. substrate (405)
2. 488/561/633
3. 458/514/561
4. 476/488
5. RT 15/85 (reflection)
6. 488/561

Galvanometric Z-stage: 

1. min. step 20 nm
2. range 1500 µm

Motorized XY scanning stage 

1. speed: 10 mm/s
2. resolution 0,02-0,04 µm
3. reproducibility

Software 

LAS X with wizard for FRET & FRAP analysis and 3D viewer

Bioluminiscence, chemiluminiscence and fluorescence (GFP)

1. resolution 1024 x 1024 px
2. sensitivity 300 to 1050 nm
3. quantum yield (QY) 90% @ 620 nm
4. dynamic range 82 dB

1. external control panel for motorized functions
2. episcopic illumination by upright semirigid LED lamps
3. transmitted light base with three types of illuminations: bright field, Rotermann contrast and dark field
4. apochromat objective 1X: NA 0,17 y WD 61,5 mm
5. apochromat objective 2X: NA 0,35 y WD 20,1 mm
6. motorized zoom, magnification between 0,78X and 16X (20,5 range)
7. motorized fluorescence route
8. Fluorescence cubes:

Features:

• Electron Multiplying Back Thinned Transfer CCD technique
• high frame rate format with 512 x 512 pixels of 16x16 μm
• readout speed of the full image 70 fps
• binning 1x1, 2x2 and 4x4
• EM (electron multiplying) gain between 1 - 1200x
• Quantum Yield (QY) > 90%
• analog gain between 0,5x and 1x for EM mode and between 1x and 5x for non-EM mode
• air (-65ºC) and water (-80ºC) cooling
• 16 bits imaging

Features:

1. CMOS sensor FL-400
2. effective number of pixels: 2048 x 2048
3. effective area: 13,312 mm x 13,312 mm
4. frame rate: 30 frames/s a máxima resolución
5. quantum yield (QY): over 70% at 600 nm
6. digital binning 2x2 / 4x4

1. touch screen to control all functions

2. metal halide lamp (120 W)

Fluorescence cubes:

Objectives:

*BF = bright field, DIC = differential interference contrast, FLUO = fluorescence

CCD camera Axiocam 503 colour

1. pixels: 1936 x 1460
2. pixel size: 4.54 μm x 4.54 μm

Confocal module

Lasers

1. 405, 488, 458, 514, 561 and 633 nm

Detectors

1. 3 spectral PMTs
2. Airyscan detector for super-resolution
3. 1 PMT for transmitted light

Motorized XY scanning stage

1. speed: 100 mm/s
2. resolution: 0,1 µm
3. reproducibility

Motorized Z stage

1. range: 500 μm
2. speed: 5 mm/sec
3. reproducibility: +/- 5 nm

Software

1. ZEN black: image acquisition
2. ZEN blue: image processing (3D, colocalisation)

Luminometer to perform screenings of immunofluorescence experiments (single or double labels), in order to select fluorescence-positive samples for subsequent observation and analysis on the confocal microscope.

Specifications:

1. Fluorescence and Luminescence..
2. FRET, TR-FRET and BRET.
3. Excitation length range: 200-1000 nm
4. Emission length range: 270-840 nm
5. Microplates from 6 to 384 wells.
6. Orbital shaking.
7. Temperature control from 4 to 45°C.

Responsable: Jaime Tome Amat - Profesor/a Contratado/a Doctor/a

Sony MA900 flow cytometer with fluorescent activated cell sorting (FACS)(S38)

Analysis and separation of cell populations.
 

1. 3 lasers: 405, 488 and 561 nm
2. 12 photomultipliers (PMT) to detect these fluorochromes: DAPI, EBFP, ECFP, EGFP, sfGFP, FITC, EYFP, tdTomato, mRFP, mCherry
3. Forward (FSC) and side (SSC) scatter channels to determine cell size and internal complexity, respectively
4. 70 y 100 μm nozzles to separate cells of different sizes
5. 2 and 4 ways separation in 1,5 ml and 5 ml, and 2 ways in 15 ml tubes
6. adaptors for plates of 6, 24, 48, 96 and 384 wells, and PCR plates
7. workstation and analysis software

Citómetro MACSQuant VYB (S38)

Citómetro de flujo para medir características de fluorescencia (entre otras) de cultivos, especialmente bacterianos. Este equipo se ubica en el Laboratorio S38 de la planta -1 (sótano) del edificio principal del CBGP y forma parte del equipamiento asociado al programa científico SynBIO2 (Biología Sintética y Bioingeniería).

El equipo MACSQuant VYB incorpora un módulo para la automatización de la recogida de un alto número de muestras, por lo que acepta la entrada de placas de 96 pocillos, con opción a su vez de entrada de un único tubo o en grupos de 24.

Dentro de las características del equipo, incluye una optimización del tipo y conjunto de fotomultiplicadores para la medición de proteínas fluorescentes expresadas intracelularmente.

Características técnicas del sistema de citometría de flujo:

1. 3 láseres de excitación: Violeta (405 nm, 65 mW), Azul (488nm, 50 mW), Amarillo (561nm, 100 mW),
2. 10 fotomultiplicadores (PMT’s) que permiten medir simultáneamente 10 parámetros ópticos (8 de fluorescencia más FSC para tamaño y SSC para complejidad celular).
3. Columna de Enriquecimiento Magnético integrada para la selección y análisis de poblaciones poco frecuentes.
4. Preparador automático integrado de muestras que permite añadir reactivos, diluir muestras, agitar e incubar, controlado por el software del citómetro.
5. Permite la toma de muestras en tubo individual (tanto tubos de 1 y 2 mL como tubos de citometría de 5, 15 y 50 mL) o racks automatizados de 24 o 96 pocillos, todo controlado por el software.
6. Permitir ciclos de encendido y apagado, además de limpieza entre muestra y muestra, automáticos.
7. Software con función de agrupamiento de datos para una mejor comparación y presentación de los gráficos.
8. Compensación automática mediante matriz de 8/14 colores.

Responsable: Miguel Ángel Moreno Risueño - Profesor/a Titular de Universidad

To determine DNA and RNA quality by automatic electrophoresis for Next Generation Sequencing analysis. 

1. determination of quality/integrity (quantity/concentration and electropherogramme) of RNA, NGS libraries, genomic DNA, cDNA
2. verification of size and quantity/concentration of PCR products
3. workstation and analysis software

Responsable: Jose Manuel Palacios Alberti - Catedratico/a de Universidad

Microbiological growth monitoring system

1. 96 wells
2. continuous shaking (lineal, orbital, doble orbital
3. incubation temperature range 15-65ºC
4. filters of 405 and 600 nm and a tunable filter between 400 and 600 nm wavelengths.
5. workstation and analysis software