- Microscopy
- Microtomes and Sample Preparation
- Upright microscope Zeiss Axiophot
- Nikon Labophot-2 vertical
- Inverted microscope Leica DM IRB
- Stereomicroscope Leica MZ10 F
- Upright microscope Leica DM2000
- Stereomicroscope Olympus SZX9
- Stereomicroscope Leica MZ95
- Spectral confocal microscope Leica TCS-SP8
- NightOwl LB 983 NC100. Berthold
- Stereomicroscope LEICA M205FA for Fluorescence and Bioluminiscence
- High sensitivity Electron Multiplying Hamamatsu ImagEM X2 EM-CCD camera
- Inverted microscope Leica DMi8
- Digital camera Hamamatsu ORCA Flash 4.0 LT
- Super-resolution confocal microscope Zeiss LSM 880 with fast Airyscan
- Luminometer Varioskan LUX
Responsable: Pablo González-Melendi de León - Profesor/a Titular de Universidad
pablo.melendi@upm.es
910679169 / 910679107 ( Lab Microscopía )
Personal:
Alonso Ramos, Paula - Técnico/a
Box mail of complaints, suggestions and congratulations
Service member of:
Spanish Network of Advanced Light Microscopy (REMOA)
Leica TCS-SP8 confocal microscope
Super-resolution confocal microscope Zeiss LSM 880 with fast Airyscan
Available equipment
Microtomes and sample preparation
- Microtome Leica HistoCore NANOCUT R
- Vibratome Leica VT1200 S
- Paraffin-wax dispenser Leica EG 1110
- Hybridisation oven Amersham
Microscopes:
- Zeiss Axiophot vertical microscope [Images]
- Leica DM IRB inverted microscope
- Leica DM2000 vertical microscope
- Stereomicroscope Leica MZ10F
- Stereomicroscope Olympus SZX9
- Stereomicroscope Leica MZ9
- Nikon Labophot-2 vertical microscope
Confocal microscope:
- Spectral confocal microscope Leica TCS-SP8 [Images]
- Super-resolution confocal microscope Zeiss LSM 880 with fast Airyscan
CCD camera
- CCD camera NightOwl LB 983 NC100. Berthold
Equipment for high sensitivity image acquisition (FLumaZone)
- Stereomicroscope LEICA M205FA for fluorescence and bioluminiscence
- High sensitivity Electron Multiplying Hamamatsu ImagEM X2 EM-CCD camera
Fluorescence motorized microscope with a high sensitive camera
- Inverted microscope Leica Dmi8
- Digital camera Hamamatsu ORCA Flash 4.0 LT
Click hereto download the Fiji software for confocal imaging management.
Microtome Leica HistoCore NANOCUT R
- Automatic or manual working mode.
- Ergonomic and independent control panel.
- Holders for paraffin-wax and resin embedded specimens.
- Holders for steel and diamond knifes.
- Minimum sectioning thickness 0,5 µm.
- Integrated stereomicroscope.
Vibratome Leica VT1200 S
- Thick sections from non-embedded specimens by blade (Guillete) vibration.
- Automatic or manual working mode.
- Ergonomic and independent control panel.
- Integrated stereomicroscope.
Paraffin-wax dispenser Leica EG 1110
Hybridisation oven Amersham
-
Fluorescence cubes:
Excitation light |
Excitation filter |
Dichroic mirror |
Emission filter |
UV |
BP 365 |
395 |
LP 397 |
blue |
450-490 |
510 |
LP 520 |
green |
BP 546 |
580 |
LP 590 |
-
Objectives:
Type |
Magnification/numerical aperture |
Technique |
Immersion |
Plan-NEOFLUAR |
2.5x/0.075 |
|
|
Plan-NEOFLUAR |
5x/0.15 |
|
|
Plan-NEOFLUAR |
10x/0.3 |
|
|
Plan-NEOFLUAR |
20x/0.5 |
DIC* |
|
Plan-NEOFLUAR |
40x/0.75 |
DIC* |
|
Plan-NEOFLUAR |
40x/1.3 |
phase contrast |
oil |
Plan-NEOFLUAR |
63x/1.25 |
|
oil |
Plan-NEOFLUAR |
100x/1.3 |
|
oil |
*DIC = differential interference contrast
Coupled to colour CCD camera Leica DFC 300FX (1,4 Mpixels)
-
Fluorescence cubes:
Type |
Excitation light |
Excitation filter |
Dichroic mirror |
Emission filter |
A |
UV |
BP 340-380 |
400 |
LP 425 |
I3 |
blue |
BP 450-490 |
510 |
LP 515 |
N2.1 |
green |
BP 515-560 |
580 |
LP 590 |
-
Objectives:
Type |
Magnification/numerical aperture |
Technique |
Immersion |
PL FLUOTAR |
1.6x/0.05 |
|
|
C PLAN |
4x/0.10 |
|
|
C PLAN |
10x/0.22 |
phase contrast |
|
N PLAN |
20x/0.40 |
|
|
N PLAN |
40x/0.55 |
phase contrast |
|
N PLAN |
100x/1.25 |
phase contrast |
oil |
- Coupled to colour CMOS camera DC.20000i euromex (20 Mpixels).
-
Fluorescence cubes:
Type |
Excitation light |
Excitation filter |
Dichroic mirror |
Emission filter |
A |
UV |
BP 340-380 |
400 |
LP 425 |
L5 |
blue |
BP 480/40 |
505 |
BP 527/30 |
N2.1 |
green |
BP 515-560 |
580 |
LP 590 |
-
Objectives:
Type |
Magnification/numerical aperture |
Technique |
Immersion |
C PLAN |
10x/0.22 |
|
|
C PLAN |
20x/0.4 |
phase contrast |
|
C PLAN |
40x/0.65 |
phase contrast |
|
HI PLAN |
63x/0.75 |
|
|
C PLAN |
100x/1.25 |
|
oil |
Coupled to colour CCD camera Leica DFC 300FX (1,4 Mpixels).
Inverted microscope Leica DMI6000CS
-
touchscreen STP6000
-
smart move (moves the stage in XY and Z)
-
metal halide bulb EL6000 (120 W)
- Fluorescence clubes:
Type |
Excitation light |
Excitation filter |
Dichroic mirror |
Emission filter |
A4 |
UV |
BP 360/40 |
400 |
BP 470/40 |
CFP |
violet/blue |
BP 436/20 |
455 |
BP 480/40 |
GFP |
blue |
BP 470/40 |
500 |
BP 525/50 |
I3 |
blue |
BP 450-490 |
510 |
LP 515 |
N2.1 |
green |
BP 515-560 |
580 |
LP 590 |
-
objectives:
Type |
Magnification/numerical aperture |
Technique* |
Immersion |
PL APO CS |
10x/0.4 |
BF,FLUO |
|
PL APO CS |
20x/0.70 |
BF,FLUO |
|
PL APO CS2 |
20X/0.75 |
BF,DIC,FLUO |
water, glycerol, oil |
PL APO CS2 |
40x/1.1 |
BF,DIC,FLUO |
water |
PL APO CS2 |
63x/1.4 |
BF,DIC,FLUO |
oil |
*BF = bright field, DIC = differential interference contrast, FLUO = fluorescence
Confocal module
FOV scanner:
- speed up to 1.800 Hz (3.600 Hz in bidirectional mode)
- up to 7 fps @ 512 x 512 px
- image format up to 8.192 x 8.192 px
Lasers:
- CW (405 nm). 50 mW
- Ar (458, 488 y 514 nm). 100 mW
- DPSS (561 nm). 20 mW
- He-Ne (633 nm). 10 mW
Detectors:
- 4 spectral for fluorescence and reflection (2 PMT and 2 HyD)
- 1 (non-confocal) for transmitted light
LIAchroic beam splitter, which includes the dichroic mirrors:
- substrate (405)
- 488/561/633
- 458/514/561
- 476/488
- RT 15/85 (reflection)
- 488/561
Galvanometric Z-stage:
- min. step 20 nm
- range 1500 µm
Motorized XY scanning stage
- speed: 10 mm/s
- resolution 0,02-0,04 µm
- reproducibility
Software
LAS X with wizard for FRET & FRAP analysis and 3D viewer
Bioluminiscence, chemiluminiscence and fluorescence (GFP)
- resolution 1024 x 1024 px
- sensitivity 300 to 1050 nm
- quantum yield (QY) 90% @ 620 nm
- dynamic range 82 dB
- external control panel for motorized functions
- episcopic illumination by upright semirigid LED lamps
- transmitted light base with three types of illuminations: bright field, Rotermann contrast and dark field
- apochromat objective 1X: NA 0,17 y WD 61,5 mm
- apochromat objective 2X: NA 0,35 y WD 20,1 mm
- motorized zoom, magnification between 0,78X and 16X (20,5 range)
- motorized fluorescence route
- Fluorescence cubes:
Type |
Excitation light |
Excitation filter |
Dichroic mirror |
DAPI |
UV |
350/50 |
460/50 |
GFP |
blue |
470/40 |
525/50 |
mCherry |
green |
560/14 |
630/75 |
Features:
- Electron Multiplying Back Thinned Transfer CCD technique
- high frame rate format with 512 x 512 pixels of 16x16 μm
- readout speed of the full image 70 fps
- binning 1x1, 2x2 and 4x4
- EM (electron multiplying) gain between 1 - 1200x
- Quantum Yield (QY) > 90%
- analog gain between 0,5x and 1x for EM mode and between 1x and 5x for non-EM mode
- air (-65ºC) and water (-80ºC) cooling
- 16 bits imaging
-
Integrated touch screen to control all functions
-
CoolLed pE300 ULTRA illumination
-
Autofocus Leica AFC (Adaptive Focus Control)
-
Fluorescence cubes:
Type |
Dye |
Excitation filter |
Dichro |
Emission filter |
LED 405 |
DAPI, BFP |
405/60 |
455 |
470/40 |
FITC/TXR |
CFP, GFP mCherry |
455/50 560/40 |
495 595 |
520/40 635/65 |
-
Objectives:
Type |
Magnification/N.A. |
Immersion |
Coverslip (mm) |
Techniques * |
HC PL Fluotar |
5x/0.15 |
|
BF, Pol, DIC, FLUO |
|
HC PL Fluotar |
10x/0.32 |
0.17 |
BF, Pol, DIC, FLUO |
|
HC PL Fluotar |
20X/0.55 |
0.17 |
BF, Pol, DIC, FLUO |
|
HC PL Fluotar |
40x/0.80 |
0.17 |
BF, Pol, DIC, FLUO |
|
HC PL APO |
63X/1,40 |
oil |
0.17 |
BF, FLUO |
*BF = bright field, DIC = differential interference contrast, Pol = polarisation, FLUO = fluorescence
XY motorized stage:
-
max. speed: 120 mm/s
-
range: 130 x 85 mm
-
resolution: 0,05 µm
- step: 10 nm
- range: 12 mm
- four speeds: 2 coarse, 2 fine
Features:
- CMOS sensor FL-400
- effective number of pixels: 2048 x 2048
- effective area: 13,312 mm x 13,312 mm
- frame rate: 30 frames/s a máxima resolución
- quantum yield (QY): over 70% at 600 nm
- digital binning 2x2 / 4x4
-
touch screen to control all functions
-
metal halide lamp (120 W)
Fluorescence cubes:
Type |
Excitation light |
Excitation filter |
Emission filter |
DAPI |
UV |
356 |
BP 455/50 |
GFP |
blue |
BP 470/40 |
BP 525/50 |
DsRed |
green |
BP 530-585 |
LP 615 |
Objectives:
Type |
Magnification/numerical aperture |
Technique* |
Inmersión |
Plan-Apochromat |
10x/0.45 |
BF,FLUO |
|
Plan-Apochromat |
25x/0.80 |
BF,DIC,FLUO |
water, glycerol, oil |
W N-Achroplan |
40x/0.75 |
BF, FLUO |
water |
Plan-Apochromat |
40X/1.2 |
BF,DIC,FLUO |
water, glycerol, oil |
Plan-Apochromat |
63x/1.4 |
BF,DIC,FLUO |
oil |
*BF = bright field, DIC = differential interference contrast, FLUO = fluorescence
CCD camera Axiocam 503 colour
- pixels: 1936 x 1460
- pixel size: 4.54 μm x 4.54 μm
Confocal module
Lasers
- 405, 488, 458, 514, 561 and 633 nm
Detectors
- 3 spectral PMTs
- Airyscan detector for super-resolution
- 1 PMT for transmitted light
Motorized XY scanning stage
- speed: 100 mm/s
- resolution: 0,1 µm
- reproducibility
Motorized Z stage
- range: 500 μm
- speed: 5 mm/sec
- reproducibility: +/- 5 nm
Software
- ZEN black: image acquisition
- ZEN blue: image processing (3D, colocalisation)

Luminometer to perform screenings of immunofluorescence experiments (single or double labels), in order to select fluorescence-positive samples for subsequent observation and analysis on the confocal microscope.
Specifications:
- Fluorescence and Luminescence..
- FRET, TR-FRET and BRET.
- Excitation length range: 200-1000 nm
- Emission length range: 270-840 nm
- Microplates from 6 to 384 wells.
- Orbital shaking.
- Temperature control from 4 to 45°C.