Microtome Leica HistoCore NANOCUT R

1. Automatic or manual working mode.
2. Ergonomic and independent control panel.
3. Holders for paraffin-wax and resin embedded specimens.
4. Holders for steel and diamond knifes.
5. Minimum sectioning thickness 0,5 µm.
6. Integrated stereomicroscope.

Vibratome Leica VT1200 S

1. Thick sections from non-embedded specimens by blade (Guillete) vibration.
2. Automatic or manual working mode.
3. Ergonomic and independent control panel.
4. Integrated stereomicroscope.

Paraffin-wax dispenser Leica EG 1110

Hybridisation oven Amersham

• Fluorescence cubes:
  
  

• Objectives:
  
  

*DIC = differential interference contrast

Coupled to colour CMOS camera DC.20000i euromex (20 Mpixels).

• Objetives:
  
  

1. diascopic and episcopic illumination
2. plan-apochromatic objective 1.0x/0.125
3. zoom 0.8x – 8x
4. fluorescence cubes
   
   

Coupled to colour CCD camera Leica DFC 400C (5 Mpixels).

• Fluorescence cubes:
  
  

• Objectives:
  
  

Coupled to colour CCD camera Leica DFC 300FX (1,4 Mpixels).

1. episcopic illumination
2. objectives 0.5x y 1.0x
3. zoom 0.63x – 5.7x
4. fluorescence cubes

1. episcopic illumination
2. objective PLAN 1.0x
3. zoom 0.63x – 6x

Inverted microscope Leica DMI6000CS 

1. touchscreen STP6000

2. smart move (moves the stage in XY and Z) 

3. metal halide bulb EL6000 (120 W)

4. Fluorescence clubes:
   
   

• objectives:
  
  

*BF = bright field, DIC = differential interference contrast, FLUO = fluorescence

Confocal module

FOV scanner: 

1. speed up to 1.800 Hz (3.600 Hz in bidirectional mode)
2. up to 7 fps @ 512 x 512 px
3. image format up to 8.192 x 8.192 px

Lasers: 

1. CW (405 nm). 50 mW
2. Ar (458, 488 y 514 nm). 100 mW
3. DPSS (561 nm). 20 mW
4. He-Ne (633 nm). 10 mW

Detectors: 

1. 4 spectral for fluorescence and reflection (2 PMT and 2 HyD)
2. 1 (non-confocal) for transmitted light

LIAchroic beam splitter, which includes the dichroic mirrors: 

1. substrate (405)
2. 488/561/633
3. 458/514/561
4. 476/488
5. RT 15/85 (reflection)
6. 488/561

Galvanometric Z-stage: 

1. min. step 20 nm
2. range 1500 µm

Motorized XY scanning stage 

1. speed: 10 mm/s
2. resolution 0,02-0,04 µm
3. reproducibility

Software 

LAS X with wizard for FRET & FRAP analysis and 3D viewer

Bioluminiscence, chemiluminiscence and fluorescence (GFP)

1. resolution 1024 x 1024 px
2. sensitivity 300 to 1050 nm
3. quantum yield (QY) 90% @ 620 nm
4. dynamic range 82 dB

1. external control panel for motorized functions
2. episcopic illumination by upright semirigid LED lamps
3. transmitted light base with three types of illuminations: bright field, Rotermann contrast and dark field
4. apochromat objective 1X: NA 0,17 y WD 61,5 mm
5. apochromat objective 2X: NA 0,35 y WD 20,1 mm
6. motorized zoom, magnification between 0,78X and 16X (20,5 range)
7. motorized fluorescence route
8. Fluorescence cubes:

Features:

• Electron Multiplying Back Thinned Transfer CCD technique
• high frame rate format with 512 x 512 pixels of 16x16 μm
• readout speed of the full image 70 fps
• binning 1x1, 2x2 and 4x4
• EM (electron multiplying) gain between 1 - 1200x
• Quantum Yield (QY) > 90%
• analog gain between 0,5x and 1x for EM mode and between 1x and 5x for non-EM mode
• air (-65ºC) and water (-80ºC) cooling
• 16 bits imaging

1. Integrated touch screen to control all functions

2. CoolLed pE300 ULTRA illumination

3. Autofocus Leica AFC (Adaptive Focus Control)

4. Fluorescence cubes:

    

• Objectives:
  
  

*BF = bright field, DIC = differential interference contrast, Pol = polarisation, FLUO = fluorescence

XY motorized stage:

1. max. speed: 120 mm/s

2. range: 130 x 85 mm

3. resolution: 0,05 µm

1. step: 10 nm
2. range: 12 mm
3. four speeds: 2 coarse, 2 fine

Features:

1. CMOS sensor FL-400
2. effective number of pixels: 2048 x 2048
3. effective area: 13,312 mm x 13,312 mm
4. frame rate: 30 frames/s a máxima resolución
5. quantum yield (QY): over 70% at 600 nm
6. digital binning 2x2 / 4x4

1. touch screen to control all functions

2. metal halide lamp (120 W)

Fluorescence cubes:

Objectives:

*BF = bright field, DIC = differential interference contrast, FLUO = fluorescence

CCD camera Axiocam 503 colour

1. pixels: 1936 x 1460
2. pixel size: 4.54 μm x 4.54 μm

Confocal module

Lasers

1. 405, 488, 458, 514, 561 and 633 nm

Detectors

1. 3 spectral PMTs
2. Airyscan detector for super-resolution
3. 1 PMT for transmitted light

Motorized XY scanning stage

1. speed: 100 mm/s
2. resolution: 0,1 µm
3. reproducibility

Motorized Z stage

1. range: 500 μm
2. speed: 5 mm/sec
3. reproducibility: +/- 5 nm

Software

1. ZEN black: image acquisition
2. ZEN blue: image processing (3D, colocalisation)

Luminometer to perform screenings of immunofluorescence experiments (single or double labels), in order to select fluorescence-positive samples for subsequent observation and analysis on the confocal microscope.

Specifications:

1. Fluorescence and Luminescence..
2. FRET, TR-FRET and BRET.
3. Excitation length range: 200-1000 nm
4. Emission length range: 270-840 nm
5. Microplates from 6 to 384 wells.
6. Orbital shaking.
7. Temperature control from 4 to 45°C.