Group leader: Araceli Díaz Perales - Professor
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Main research lines of Plant Allergens group:

  1. Molecular basis of allergenicity of plant food allergy.
  2. Interactions of allergens with mucosa and immune cells.
  3. Production of new diagnostic methods by generating allergen microarrays.
  4. Design of new strategies for treatment of allergies through production of hypoallergenic forms.


The Plant Allergens group is involved in the Spanish Allergy Health Network (RETIC-Programme Carlos III Institute) and has been studying the molecular basis of plant allergy for over 20 years, characterizing allergens, and improving their diagnosis and treatment.



Our research activity has been following in recent years two different but complementary strategies. We use techniques and methods from Biochemistry, Molecular Biology, and Immunology to identify and characterize the molecular mechanisms underlying the allergic response.


We also resort to computational methods to study the physicochemical and structural properties of allergens. Docking analyses, electrostatic potentials, Molecular Mechanics calculations and Molecular Dynamics simulations have been employed to investigate modes of protein-ligand binding and oligomerization states of allergens.



This dual approach has been applied to deepen our knowledge on the molecular basis of allergenicity using two model, prototypical allergens such as Pru p 3 (the major allergen from peach) and Alt a 1 (the principal allergen of Alternaria fungi associated to chronic asthma).


In the search for common denominators for allergenicity, the presence of ligands carried by allergen proteins has been found to play a key role in the sensitization phase of allergic disease.

A tale of two allergens

Pru p 3

What is the role of an allergen in plant?

Pru p 3 is located on the stigma of pollinated flower and its localization and timing expression suggest a role in flowering linked to pollination.




 Pru p 3 is also expressed in a very limited time period in peel fruit. The protein is primarily localized in trichome secretory cells during fruit development. So, Pru p 3 may be involved in processes to inhibit second pollination in flower and herbivore feeding until seed is completely developed.

What is carried by an allergen?

The native ligand of Pru p 3 is a derivative of the alkaloid camptothecin bound to a long hydrophobic tail of phytosphingosine.

This ligand inhibits topoisomerase I activity, as camptothecin does, and also participates actively in maturation of antigen-presenting cells by a TLR4-mediated mechanism.

The ligand also seems the responsible for the immune capacity of the peach allergen Pru p 3.




Alt a 1

Where is a fungal allergen in infected fruit?


Alt a 1 is firstly localized inside ungerminated fungal spores by immunofluorescence with specific monoclonal antibody. The most intense signal is observed in the spore wall. Inoculating kiwifruits with isolated spores from Alternaria, no hyphae development occurs until 14 dpi.




However, even though hyphae are not observed, the presence of the allergen is confirmed in the kiwi pulp after 7 dpi.


 What happens when Alt a 1 induces an allergic response in humans?

Combining in vitro experimental and in silico computational results it is possible to track the molecular events that occur when Alternaria spores arrive at bronchial epithelium in humans. Different oligomerization states, the presence of a flavonol ligand, effects of variations of pH, recognition by cell receptors and interaction with defense proteins such as human LCN2 are pieces that come from those results. These pieces fit together to provide a picture on the molecular mechanism of the inflammatory response induced by Alt a 1 that leads to activation of immune cells and imbalance towards a Th2 response.



  1. Authors: Araceli Díaz Perales, Cristina Gómez Casado, Luis Fernández Pacios (UPM) Josef Singer1, Erika Jensen-Jarolim1, Franziska Roth-Walter 2 (1 Medical University of Vienna, 2 Veterinary Medicine University of Vienna)
  2. Title: Methods and means for diagnosing and treating allergy
  3. Type: European Patent
  4. Reference: EP14150965.3
  5. Priority date: 13-01-14
  6. Authors:Araceli Díaz Perales, Luis Fernández Pacios (UPM) Cristobalina Mayorga Mayorga 1, Mª José Torres Jaen 1, Miguel Blanca Gómez 1, Francisco Javier Rojo Marcos 2, Ainhoa Mascaraque González 2, Francisco Javier Ramos Soriano 2, David Andreu Martínez 3, Javier Valle García 3 (1 Servicio Andaluz de Salud, 2 CSIC, 3 Universitat Pompeu Fabra)
  7. Title: Composición para el tratamiento de la alergia
  8. Type: Patente Española
  9. Reference: P201531341
  10. Priority date: 21-09-15


Representative Publications

Mínguez-Toral, M., Pacios, L.F., Sánchez, F., Ponz, F. 2023. Structural intrinsic disorder in a functionalized potyviral coat protein as a main viability determinant of its assembled nanoparticles. International Journal of Biological Macromolecules 236, 123958. DOI: 10.1016/j.ijbiomac.2023.123958

Martín-Dacal, M., Fernández-Calvo, P., Jiménez-Sandoval, P., López, G., Garrido-Arandía, M., Rebaque, D., del Hierro, I., Berlanga, D.J., Torres, M.Á., Kumar, V., Mélida, H., Pacios, L.F., Santiago, J., Molina, A. 2022. Arabidopsis immune responses triggered by cellulose and mixed-linked glucan oligosaccharides require a group of Leucine-Rich Repeat-Malectin Receptor Kinases. The Plant Journal. DOI: 10.1111/tpj.16088

Dijk, W., Villa, C., Benedé, S., Vassilopoulou, E., Mafra, I., Garrido-Arandia, M., Martínez Blanco, M., Bouchaud, G., Hoppenbrouwers, T., Bavaro, S.L., Giblin, L., Knipping, K., Castro, A.M., Delgado, S., Costa, J., Bastiaan-Net, S. 2022. Critical features of an in vitro intestinal absorption model to study the first key aspects underlying food allergen sensitization. Comprehensive Reviews in Food Science and Food Safety. DOI: 10.1111/1541-4337.13097

Meile, L., Garrido-Arandia, M., Bernasconi, Z., Peter, J., Schneller, A., Bernasconi, A., Alassimone, J., McDonald, B.A., Sánchez-Vallet, A. 2022. Natural variation in Avr3D1 from Zymoseptoria sp. contributes to quantitative gene-for-gene resistance and to host specificity. New Phytologist. DOI: 10.1111/nph.18690

Regner, A., Szepannek, N., Wiederstein, M., Fakhimahmadi, A., Pacios, L.F., Blokhuis, B.R., Redegeld, F.A., Hofstetter, G., Dvorak, Z., Jensen-Jarolim, E., Hufnagl, K., Roth-Walter, F. 2023. Binding to Iron Quercetin Complexes Increases the Antioxidant Capacity of the Major Birch Pollen Allergen Bet v 1 and Reduces Its Allergenicity. Antioxidants 12, 42. DOI: 10.3390/antiox12010042

Pazos-Castro, D., Margain, C., Gonzalez-Klein, Z., Yuste-Calvo, C., Garrido-Arandia, M., Zurita, L., Esteban, V., Tome-Amat, J., Diaz-Perales, A., Ponz, F. 2022. Suitability of potyviral recombinant virus-like particles bearing a complete food allergen for immunotherapy vaccines. Frontiers in Immunology 13. DOI: 10.3389/fimmu.2022.986823

Murillo, A.M.M., Valle, L.G., Ramírez, Y., Sánchez, M.J., Santamaría, B., Molina-Roldan, E., Ortega-Madueño, I., Urcelay, E., Tramarin, L., Herreros, P., Díaz-Perales, A., Garrido-Arandia, M., Tome-Amat, J., Hernández-Ramírez, G., Espinosa, R.L., Laguna, M.F., Holgado, M. 2022. Integration of Multiple Interferometers in Highly Multiplexed Diagnostic KITs to Evaluate Several Biomarkers of COVID-19 in Serum. Biosensors 12, 671. DOI: 10.3390/bios12090671

Diem, L., Neuherz, B., Rohrhofer, J., Koidl, L., Asero, R., Brockow, K., Diaz Perales, A., Faber, M., Gebhardt, J., Torres, M., Jensen-Jarolim, E., Zehetmayer, S., Untersmayr, E. n.d. Real-life evaluation of molecular multiplex IgE test methods in the diagnosis of pollen associated food allergy. Allergy n/a. DOI: 10.1111/all.15329

Missaoui, K., Gonzalez-Klein, Z., Jemli, S., Garrido-Arandia, M., Diaz-Perales, A., Tome-Amat, J., Brini, F. 2022. Identification and molecular characterization of a novel non-specific lipid transfer protein (TdLTP2) from durum wheat. PLOS ONE 17, e0266971. DOI: 10.1371/journal.pone.0266971

Pali-Schöll, I., Bianchini, R., Afify, S.M., Hofstetter, G., Winkler, S., Ahlers, S., Altemeier, T., Mayerhofer, H., Hufnagl, K., Korath, A.D.J., Pranger, C., Widhalm, R., Hann, S., Wittek, T., Kasper-Giebl, A., Pacios, L.F., Roth-Walter, F., Vercelli, D., von Mutius, E., Jensen-Jarolim, E. 2022. Secretory protein beta-lactoglobulin in cattle stable dust may contribute to the allergy-protective farm effect. Clinical and Translational Allergy 12, e12125. DOI: 10.1002/clt2.12125

Pazos-Castro, D., Gonzalez-Klein, Z., Montalvo, A.Y., Hernandez-Ramirez, G., Romero-Sahagun, A., Esteban, V., Garrido-Arandia, M., Tome-Amat, J., Diaz-Perales, A. 2022. NLRP3 priming due to skin damage precedes LTP allergic sensitization in a mouse model. Scientific Reports 12, 3329. DOI: 10.1038/s41598-022-07421-y

Missaoui, K., Gonzalez-Klein, Z., Pazos-Castro, D., Hernandez-Ramirez, G., Garrido-Arandia, M., Brini, F., Diaz-Perales, A., Tome-Amat, J. 2022. Plant non-specific lipid transfer proteins: An overview. Plant Physiology and Biochemistry 171, 115–127. DOI: 10.1016/j.plaphy.2021.12.026

Hernandez-Ramirez, G., Barber, D., Tome-Amat, J., Garrido-Arandia, M., Diaz-Perales, A. 2021. Alternaria as an Inducer of Allergic Sensitization. Journal of Fungi 7, 838. DOI: 10.3390/jof7100838

Phillips, A.H., Hernandez, J.A., Payá-Tormo, L., Burén, S., Cuevas-Zuviría, B., Pacios, L.F., Pelton, J.G., Wemmer, D.E., Rubio, L.M. 2021. Environment and coordination of FeMo–co in the nitrogenase metallochaperone NafY. RSC Chemical Biology. DOI: 10.1039/D1CB00086A

Murillo, A.M.M., Tomé-Amat, J., Ramírez, Y., Garrido-Arandia, M., Valle, L.G., Hernández-Ramírez, G., Tramarin, L., Herreros, P., Santamaría, B., Díaz-Perales, A., Holgado, M. 2021. Developing an optical interferometric detection method based biosensor for the detection of detecting specific SARS-CoV-2 immunoglobulins in serum and saliva, and their corresponding ELISA correlation. Sensors and Actuators B: Chemical 130394. DOI: 10.1016/j.snb.2021.130394

Skypala, I.J., Asero, R., Barber, D., Cecchi, L., Diaz-Perales, A., Hoffmann-Sommergruber, K., Pastorello, E.A., Swoboda, I., Bartra, J., Ebo, D.G., Faber, M.A., Fernández-Rivas, M., Gomez, F., Konstantinopoulos, A.P., Luengo, O., Ree, R. van, Scala, E., Till, S.J. 2021. Non-specific lipid-transfer proteins: Allergen structure and function, cross-reactivity, sensitization, and epidemiology. Clinical and Translational Allergy 11, e12010. DOI: 10.1002/clt2.12010

Gonzalez-Klein, Z., Cuevas-Zuviria, B., Wangorsch, A., Hernandez-Ramirez, G., Pazos-Castro, D., Oeo-Santos, C., Romero-Sahagun, A., Pacios, L.F., Tome-Amat, J., Scheurer, S., Diaz-Perales, A., Garrido-Arandia, M. 2021. The key to the allergenicity of lipid transfer protein (LTP) ligands: A structural characterization. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids 1866, 158928. DOI: 10.1016/j.bbalip.2021.158928

Cuevas-Zuviría, B., Pacios, L.F. 2021. Machine Learning of Analytical Electron Density in Large Molecules Through Message-Passing. Journal of Chemical Information and Modeling. DOI: 10.1021/acs.jcim.1c00227

Skypala, I.J., Bartra, J., Ebo, D.G., Faber, M.A., Fernández‐Rivas, M., Gomez, F., Luengo, O., Till, S.J., Asero, R., Barber, D., Cecchi, L., Diaz-Perales, A., Hoffmann‐Sommergruber, K., Pastorello, E.A., Swoboda, I., Konstantinopoulos, A.P., Ree, R. van, Scala, E. 2021. The diagnosis and management of allergic reactions in patients sensitized to non-specific Lipid Transfer Proteins. Allergy. DOI:

Costa, J., Villa, C., Verhoeckx, K., Cirkovic-Velickovic, T., Schrama, D., Roncada, P., Rodrigues, P.M., Piras, C., Martín-Pedraza, L., Monaci, L., Molina, E., Mazzucchelli, G., Mafra, I., Lupi, R., Lozano-Ojalvo, D., Larré, C., Klueber, J., Gelencser, E., Bueno-Diaz, C., Diaz-Perales, A., Benedé, S., Bavaro, S.L., Kuehn, A., Hoffmann-Sommergruber, K., Holzhauser, T. 2021. Are Physicochemical Properties Shaping the Allergenic Potency of Animal Allergens?. Clinical Reviews in Allergy & Immunology. DOI: 10.1007/s12016-020-08826-1

Espinosa, R.L., Garrido-Arandia, M., Romero-Sahagun, A., Herreros, P., Tramarin, L., Laguna, M.F., Díaz-Perales, A., Holgado, M. 2020. A new optical interferometric-based in vitro detection system for the specific IgE detection in serum of the main peach allergen. Biosensors and Bioelectronics 169, 112641. DOI: 10.1016/j.bios.2020.112641

Costa, J., Bavaro, S.L., Benedé, S., Diaz-Perales, A., Bueno-Diaz, C., Gelencser, E., Klueber, J., Larré, C., Lozano-Ojalvo, D., Lupi, R., Mafra, I., Mazzucchelli, G., Molina, E., Monaci, L., Martín-Pedraza, L., Piras, C., Rodrigues, P.M., Roncada, P., Schrama, D., Cirkovic-Velickovic, T., Verhoeckx, K., Villa, C., Kuehn, A., Hoffmann-Sommergruber, K., Holzhauser, T. 2020. Are Physicochemical Properties Shaping the Allergenic Potency of Plant Allergens?. Clinical Reviews in Allergy & Immunology. DOI: 10.1007/s12016-020-08810-9

Cuevas-Zuviría, B., Pacios, L.F. 2020. Analytical Model of Electron Density and Its Machine Learning Inference. Journal of Chemical Information and Modeling 60, 3831–3842. DOI: 10.1021/acs.jcim.0c00197

Mínguez-Toral, M., Cuevas-Zuviría, B., Garrido-Arandia, M., Pacios, L.F. 2020. A computational structural study on the DNA-protecting role of the tardigrade-unique Dsup protein. Scientific Reports 10, 13424. DOI: 10.1038/s41598-020-70431-1

López‐González, S., Navarro, J.A., Pacios, L.F., Sardaru, P., Pallás, V., Sánchez, F., Ponz, F. 2020. Association between flower stalk elongation, an Arabidopsis developmental trait, and the subcellular location and movement dynamics of the nonstructural protein P3 of Turnip mosaic virus. Molecular Plant Pathology. DOI: 10.1111/mpp.12976

Roth-Walter, F., Afify, S.M., Pacios, L.F., Blokhuis, B.R., Redegeld, F., Regner, A., Petje, L.-M., Fiocchi, A., Untersmayr, E., Dvorak, Z., Hufnagl, K., Pali-Schöll, I., Jensen-Jarolim, E. 2020. Cow’s milk protein β-lactoglobulin confers resilience against allergy by targeting complexed iron into immune cells. Journal of Allergy and Clinical Immunology. DOI: 10.1016/j.jaci.2020.05.023

Hernández‐Ramírez, G., Pazos‐Castro, D., Torrijos, E.G., Montalvo, A.Y., Romero‐Sahagun, A., González‐Klein, Z., Jimeno‐Nogales, L., Escribese, M.M., Ortega, A.E., Nuñez‐Borque, E., Orvay, L.B., Esteban, V., Brito, F.F., Barber, D., Tome‐Amat, J., Garrido‐Arandia, M., Díaz‐Perales, A. 2020. Group 1 allergens, transported by mold spores, induce asthma exacerbation in a mouse model. Allergy. DOI: 10.1111/all.14347

Cuevas-Zuviría, B., Mínguez-Toral, M., Díaz-Perales, A., Garrido-Arandia, M., Pacios, L.F. 2020. Structural Dynamics of the Lipid Antigen-Binding Site of CD1d Protein. Biomolecules 10, 532. DOI: 10.3390/biom10040532

Cuevas-Zuviría, B., Mínguez-Toral, M., Díaz-Perales, A., Garrido-Arandia, M., Pacios, L.F. 2020. Dynamic plasticity of the lipid antigen-binding site of CD1d is crucially favoured by acidic pH and helper proteins. Scientific Reports 10, 5714. DOI: 10.1038/s41598-020-62833-y

Blanca, M., Puche, L.V., Garrido-Arandia, M., Martin-Pedraza, L., Sahagún, A.R., López-Sánchez, J.D., Galán, C., Marin, A., Villaba, M., Díaz-Perales, A., Somoza, M.L. 2020. Pru p 9, a new allergen eliciting respiratory symptoms in subjects sensitized to peach tree pollen. PLOS ONE 15, e0230010. DOI: 10.1371/journal.pone.0230010

Orozco-Navarrete, B., Kaczmarska, Z., Dupeux, F., Garrido-Arandia, M., Pott, D., Díaz-Perales, A., Casañal, A., Márquez, J.A., Valpuesta, V., Merchante, C. 2019. Structural Bases for the Allergenicity of Fra a 1.02 in Strawberry Fruits. Journal of Agricultural and Food Chemistry. DOI: 10.1021/acs.jafc.9b05714

Oeo‐Santos, C., Navas, A., Benedé, S., Ruíz‐León, B., Díaz‐Perales, A., Vogel, L., Moreno‐Aguilar, C., Jurado, A., Villalba, M., Barderas, R. 2019. New insights into the sensitization to non-related nsLTPs from pollen and food: new role of the allergen Ole e 7. Allergy. DOI: 10.1111/all.14086

Victorio Puche, L., Somoza, M.L., López-Sánchez, J.D., Garrido-Arandia, M., Díaz-Perales, A., Blanca, M. 2019. Peach Tree Pollen and Prunus persica 9 Sensitisation and Allergy in Children and Adolescents. International Archives of Allergy and Immunology 180, 212–220. DOI: 10.1159/000502352

Delgado, R., Abad-Guamán, R., Nicodemus, N., Diaz-Perales, A., García, J., Carabaño, R., Menoyo, D. 2019. Effect of pre- and post-weaning dietary supplementation with arginine and glutamine on rabbit performance and intestinal health. BMC Veterinary Research 15, 199. DOI: 10.1186/s12917-019-1945-2

Garrido‐Arandia, M., Tome‐Amat, J., Pazos‐Castro, D., Esteban, V., Escribese, M.M., Hernández‐Ramírez, G., Yuste, A.M., Barber, D., Pacios, L.F., Díaz‐Perales, A. 2019. Interaction of Alt a 1 with SLC22A17 in the airway mucosa. Allergy. DOI: 10.1111/all.13877

Somoza, M.L., Garrido‐Arandia, M., Victorio, L., López‐Sánchez, J.D., Blanca‐López, N., Haroun‐Diaz, E., Sahagún, A.R., Díaz‐Perales, A., Canto, G., Blanca, M. 2019. Peach tree pollen and Pru p 9 may induce rhinoconjunctivitis and asthma in children. Pediatric Allergy and Immunology. DOI: 10.1111/pai.13067

Yuste-Calvo, C., González-Gamboa, I., Pacios, L.F., Sánchez, F., Ponz, F. 2019. Structure-Based Multifunctionalization of Flexuous Elongated Viral Nanoparticles. ACS Omega 4, 5019–5028. DOI: 10.1021/acsomega.8b02760

Cuevas-Zuviría, B., Garrido-Arandia, M., Díaz-Perales, A., Pacios, L.F. 2019. Energy Landscapes of Ligand Motion Inside the Tunnel-Like Cavity of Lipid Transfer Proteins: The Case of the Pru p 3 Allergen. International Journal of Molecular Sciences 20, 1432. DOI: 10.3390/ijms20061432

Lozano-Ojalvo, D., Benedé, S., Antunes, C.M., Bavaro, S.L., Bouchaud, G., Costa, A., Denery-Papini, S., Díaz-Perales, A., Garrido-Arandia, M., Gavrovic-Jankulovic, M., Hayen, S., Martínez-Blanco, M., Molina, E., Monaci, L., Pieters, R.H.H., Villemin, C., Wichers, H.J., Wróblewska, B., Willemsen, L.E.M., Roggen, E.L., van Bilsen, J.H.M. 2019. Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies. Trends in Food Science & Technology 85, 307–319. DOI: 10.1016/j.tifs.2019.01.014

Albareda, M., Pacios, L.F., Palacios, J.M. 2019. Computational analyses, molecular dynamics, and mutagenesis studies of unprocessed form of [NiFe] hydrogenase reveal the role of disorder for efficient enzyme maturation. Biochimica et Biophysica Acta (BBA) - Bioenergetics 1860, 325–340. DOI: 10.1016/j.bbabio.2019.01.001

Rodriguez, M.J., Palomares, F., Bogas, G., Torres, M.J., Diaz‐Perales, A., Rojo, J., Plaza‐Seron, M. del C., Rodriguez‐Nogales, A., Orengo, C., Mayorga, C., Perkins, J.R. 2018. Transcriptional profiling of dendritic cells in a mouse model of food-antigen induced anaphylaxis reveals the upregulation of multiple immune-related pathways. Molecular Nutrition & Food Research 1800759. DOI: 10.1002/mnfr.201800759

Mazzucchelli, G; Holzhauser, T; Cirkovic Velickovic, T; Diaz-Perales, A; Molina, E; Roncada, P; Rodrigues, P; Verhoeckx, K; Hoffmann-Sommergruber, K. 2018. "Current (food) allergenic risk assessment: Is it fit for novel foods? Status Quo and identification of gaps". Molecular Nutrition and Food Research. DOI: 10.1002/mnfr.201700278".

Safi, H; Wangorsch, A; Lidholm, J; Brini, F; Spiric, J; Rihs, H-P; Vieths, S; Armentia, A; Farioli, L; Diaz-Perales, A; Pastorello, EA; Scheurer, S. "Identification and molecular characterization of allergenic nsLTP from durum wheat (Triticum turgidum)". Clinical & Experimental Allergy. DOI: 10.1111/cea.13271".

Gómez-Gómez, L; Pacios, LF; Diaz-Perales, A; Garrido-Arandia, M; Argandoña, J; Rubio-Moraga, Á; Ahrazem, O. 2018. "Expression and interaction analysis among saffron ALDHs and crocetin dialdehyde". International Journal of Molecular Sciences. DOI: 10.3390/ijms19051409".

Palomares, F; Gomez, F; Bogas, G; Campo, P; Perkins, JR; Diaz-Perales, A; Rodriguez, MJ; Prieto, A; Barber, D; Torres, MJ; Mayorga, C. 2018. "Immunological changes induced in peach allergy patients with systemic reactions by Pru p 3 sublingual immunotherapy". Molecular Nutrition & Food Research. DOI: 10.1002/mnfr.201700669".

Garrido-Arandia, M; Cuevas-Zuviria, B; Diaz-Perales, A; Pacios, LF. 2018. "A comparative study of human saposins". Molecules. DOI: 10.3390/molecules23020422".

Hufnagl, K; Ghosh, D; Wagner, S; Fiocchi, A; Dahdah, L; Bianchini, R; Braun, N; Steinborn, R; Hofer, M; Blaschitz, M; Roth, GA; Hofstetter, G; Roth-Walter, F; Pacios, LF; Jensen-Jarolim, E. 2018. "Retinoic acid prevents immunogenicity of milk lipocalin Bos d 5 through binding to its immunodominant T-cell epitope". Scientific Reports. DOI: 10.1038/s41598-018-19883-0".

Roth-Walter, F; Pacios, LF; Bianchini, R; Jensen-Jarolim, E. 2017. "Linking iron-deficiency with allergy: role of molecular allergens and the microbiome". Metallomics. DOI: 10.1039/c7mt00241f".

Mélida, H; Sopeña-Torres, S; Bacete, L; Garrido-Arandia, M; Jordá, L; López, G; Muñoz, A; Pacios, LF; Molina, A. 2017. "Non-branched β-1,3-glucan oligosaccharides trigger immune responses in Arabidopsis". Plant Journal. DOI: 10.1111/tpj.13755".

Tordesillas, L; Cubells-Baeza, N; Gómez-Casado, C; Berin, C; Esteban, V; Barcik, W; O'Mahony, L; Ramirez, C; Pacios, LF; Garrido-Arandia, M; Díaz-Perales, A. 2017. "Mechanisms underlying induction of allergic sensitization by Pru p 3". Clinical & Experimental Allergy. DOI: 10.1111/cea.12962".

Mas, S; Oeo-Santos, C; Cuesta-Herranz, J; Diaz-Perales, A; Colas, C; Fernandez, J; Barber, D; Rodriguez, R; de Los Rios, V; Barderas, R; Villalba, M. 2017. "A relevant IgE-reactive 28kDa protein identified from Salsola kali pollen extract by proteomics is a natural degradation product of an integral 47kDa polygalaturonase". Biochimica et Biophysica Acta. DOI: 10.1016/j.bbapap.2017.05.007".

Benedé, S; Garrido-Arandia, M; Martín-Pedraza, L; Bueno, C; Díaz-Perales, A; Villalba, M. 2017. "Multifactorial modulation of food-induced anaphylaxis". Frontiers in Immunology. DOI: 10.3389/fimmu.2017.00552".

Cubells-Baeza, N; Gómez-Casado, C; Tordesillas, L; Ramírez-Castillejo, C; Garrido-Arandia, M; González-Melendi, P; Herrero, M; Pacios, LF; Díaz-Perales, A. 2017. "Identification of the ligand of Pru p 3, a peach LTP". Plant Molecular Biology. DOI: 10.1007/s11103-017-0590-z".

Gomez, F; Bogas, G; Gonzalez, M; Campo, P; Salas, M; Diaz-Perales, A; Rodriguez, MJ; Prieto, A; Barber, D; Blanca, M; Torres, MJ; Mayorga, C. 2017. "The clinical and immunological effects of Pru p 3 SLIT on peach and peanut allergy in patients with systemic reactions". Clinical & Experimental Allergy. DOI: 10.1111/cea.12901".

Lavilla, M; Orcajo, J; Díaz-Perales, A; Gamboa, P. 2016. "Examining the effect of High Pressure Processing on the allergenic potential of the major allergen in peach (Pru p 3)". Innovative Food Science & Emerging Technologies. DOI: 10.1016/j.ifset.2016.06.021".

Gómez-Casado, C; Díaz-Perales, A. 2016. "Allergen-associated immunomodulators: modifying allergy outcome". Archivum Immunologiae et Therapiae Experimentalis. DOI: 10.1007/s00005-016-0401-2".

Cubells-Baeza, N; Verhoeckx, KCM; Larre, C; Denery-Papini, S; Gavrovic-Jankulovic, M; Diaz Perales, A. 2016. "Applicability of epithelial models in protein permeability/transport studies and food allergy". Drug Discovery Today: Disease Models. DOI: 10.1016/j.ddmod.2016.08.002".

Garrido-Arandia, M; Silva-Navas, J; Ramírez-Castillejo, C; Cubells-Baeza, N; Gómez-Casado, C; Barber, D; Pozo, JC; Melendi, PG; Pacios, LF; Díaz-Perales, A. 2016. "Characterisation of a flavonoid ligand of the fungal protein Alt a 1". Scientific Reports. DOI: 10.1038/srep33468".

Poulicard, N; Pacios, LF; Gallois, J-L; Piñero, D; García-Arenal, F. 2016. "Human management of a wild plant modulates the evolutionary dynamics of a gene determining recessive resistance to virus infection". PLoS Genetics. DOI: 10.1371/journal.pgen.1006214".

Pastor-Vargas, C; Maroto, AS; Díaz-Perales, A; Villalba, M; Esteban, V; Ruiz-Ramos, M; Rodriguez de Alba, M; Vivanco, F; Cuesta-Herranz, J. 2016. "Detection of major food allergens in amniotic fluid: initial allergenic encounter during pregnancy". Pediatric Allergy and Immunology. DOI: 10.1111/pai.12608".

Garrido-Arandia, M; Bretones, J; Gómez-Casado, C; Cubells, N; Díaz-Perales, A; Pacios, LF. 2016. "Computational study of pH-dependent oligomerization and ligand binding in Alt a 1, a highly allergenic protein with a unique fold". Journal of Computer-Aided Molecular Design. DOI: 10.1007/s10822-016-9911-6".

Pascal, M; Muñoz-Cano, R; Milà, J; Sanz, ML; Diaz-Perales, A; Sánchez-López, J; García-Moral, A; Juan, M; Valero, A; Yagüe, J; Picado, C; Bartra, J. 2016. "Nonsteroidal anti-inflammatory drugs enhance IgE-mediated activation of human basophils in patients with food anaphylaxis dependent and independent of nonsteroidal anti-inflammatory drugs". Clinical & Experimental Allergy. DOI: 10.1111/cea.12735".

Goikoetxea, M; D’Amelio, C; Martínez-Aranguren, R; Gamboa, P; Garcia, B; Gómez, F; Fernández, J; Bartra, J; Parra, A; Alvarado, M; Alonso, M; González, E; Terrados, S; Moya, C; Blanca, N; Feo-Brito, F; Villalba, M; Díaz-Perales, A; Sanz, M. 2016. "Is Microarray Analysis Really Useful and Sufficient to Diagnose Nut Allergy in the Mediterranean Area?". Journal of Investigational Allergology and Clinical Immunology. DOI: 10.18176/jiaci.0005".

Martín-Muñoz, MF; Rivero, D; Díaz Perales, A; Polanco, I; Quince, S. 2016. "Wheat allergy in celiac children". Pediatric Allergy and Immunology. DOI: 10.1111/pai.12487".

Jensen-Jarolim, E; Pacios, LF; Bianchini, R; Hofstetter, G; Roth-Walter, F. 2015. "Structural similarities of human & mammalian lipocalins, and their function in innate immunity and allergy". Allergy. DOI: 10.1111/all.12797".

Mas, S; Boissy, P; Monsalve, RI; Cuesta-Herranz, J; Díaz-Perales, A; Fernández, J; Colás, C; Rodríguez, R; Barderas, R; Villalba, M. 2015. "A recombinant Sal k 1 isoform as an alternative to the polymorphic allergen from Salsola kali pollen for allergy diagnosis". International Archives of Allergy and Immunology. DOI: 10.1159/000434680".

Albareda, M.; Pacios, L.F.; Manyani, H.; Rey, L.; Brito, B.; Imperial, J.; Ruiz-Argueso, T.; Palacios, J.M. 2014. "Maturation of Rhizobium leguminosarum hydrogenase in the presence of oxygen requires the interaction of the chaperone HypC and the scaffolding protein HupK". Journal of Biological Chemistry 289, 21217-21229. DOI: 10.1074/jbc.M114.577403".

Fraile, A; Hily, J-M; Pagán, I; Pacios, LF; García-Arenal, F. 2014. "Host resistance selects for traits unrelated to resistance-breaking that affect fitness in a plant virus". Molecular Biology and Evolution. DOI: 10.1093/molbev/msu045".

Gómez-Casado, C; Roth-Walter, F; Jensen-Jarolim, E; Díaz-Perales, A; Pacios, LF. 2013. "Modeling iron-catecholates binding to NGAL protein". Journal of Molecular Graphics & Modelling. DOI:".