Group leader: Araceli Díaz Perales - Professor
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Main research lines of the Plant Allergens group:

  1. Molecular basis of allergenicity of plant food allergens.
  2. Interactions of allergens with mucosa and immune cells.
  3. Development of novel diagnostic methods by generating allergen microarrays.
  4. Design of new strategies for allergy treatment based on VLPs.

The Plant Allergens group (alislab151) is studying molecular basis of allergy for over 20 years, characterizing plant allergens, and improving their diagnosis and treatment. Our research follows two complementary strategies combining techniques and methods from Biochemistry, Molecular Biology, and Immunology to identify and characterize the molecular mechanisms of the allergic response, with computational methods to study the physicochemical and structural properties of allergens. Docking analyses, Molecular Mechanic Calculations and Molecular Dynamics simulations are employed to model protein-ligand binding and oligomerization states of allergens.



Why do we work on food allergy in a plant center like the CBGP?

Because many plant allergens are plant defense proteins (Table). Therefore, modified plants, more resistant to climate change, with less nutritional requirements, etc., might increase the quantity of allergens, with the concomitant risk for public health.




Plant lipid transfer proteins (LTPs) are the major allergens in the Mediterranean area

Allergic sensitization is a complex process developing a heightened immune response to otherwise harmless substances, known as allergens. Identify the contributors to this response is crucial to develop effective prevention and treatment strategies.

Our lab studies the implication of the lipid ligand carried by some plant pathogenesis-related proteins (PRs) in allergic develpement. LTPs, main food allergens, carry a specific ligand consisting on a polar head derived from camptothecin (CPT), linked through an amide bond to a phytosphingosine tail (PHS). By using Pru p 3, peach allergen, as a model, we have shown its relevant role in allergic sensitization.


Working with five allergenic LTPs (Pru p 3 (as model), Tri a 14, Art v 3, Par j 2, and Ole e 7), our lab demonstrated that they transport a ligand with the same chemical identity. Further characterization of the ligand showed the role of the polar head and phytosphingosine tail in sensitization, being a critical factor in this process.


To show the immunological role of the LTPs’ ligand, two non-LTP-proteins, bovine serum albumin (BSA) and ovalbumin (OVA), were used in an allergic mouse model in complex with it. Computational analysis predicted BSA to bind five molecules of LTPs’ ligand, and OVA, only bound (A), which was confirmed in-vitro (B). Both complexes (BSA-ligand and OVA-ligand) promoted immune response on THP1-XBlue cells (C).


Moreover, our lab developed allergic mouse models using two different strains (C3H and BALB/c) exposed to both complexes as skin sensitizers (D). Only mice sensitized with Protein-LTPs’_ligand complex suffered a drop in temperature after challenge (E), confirming the relevance of the LTPs’ ligand in the allergic sensitization process.



Improving the food allergy diagnosis: Point-of-care device

The AllerScreening project ( has focused on translating an optical diagnostic technology and its read-out-platform (MOX), to the clinical routine, addressing a healthcare need. This novel, multiplexed, innovative, cost-effective system for the diagnosis of food allergies help clinicians to detect food allergies through a simple test using sera by a competitive cost. The technology developed is positioned in a TRL6, that is, the AllerScreening system has been proved in a real scenario like the final ones in case it will be upgraded to TRL9.


Working to prevent unwanted encounters: searching for hidden allergens

With no curative treatment for food allergies, it is essential to develop effective methods for their detection. Portable devices represent a promising solution to enable patients to make informed decisions about their diet in real time.

In the FOOD-AL Project, a Dot-Blot device was developed to detect specific allergens from 4 foods (Fig), with high sensitivity (1-5 ppm). Our lab is now immerse in the development of a multiplexed device based on lateral flow, which will be more user-friendly and cost-effective.



The search for definitive treatments for food allergy

In the search for a food allergy treatment, our lab makes use of VLPs by coupling TuMV-derived VLPs to Pru p 3. VLPs-Pru p 3 are produced in Nicotiana benthamiana plants and analyzed by EM and DLS (Figure).



The IT-formula was studied as therapy in a mouse model of peach allergy (A). Sublingual administration of the IT-formula reduced serological markers associated with allergic responses in 30% of the mice, such as anti-Prup3_sIgE (B-D), and reduced leukocyte infiltration in treated mice to similar levels as controls (E-F). Noteworthy, no associated macroscopic, nephritic, or hepatic toxicity was detected, showing the safety of the IT-formula. Our lab keeps developing and optimizing the formula in the search of an effective treatment of food allergy.




Representative Publications

Truchado, D.A., Juárez-Molina, M., Rincón, S., Zurita, L., Tomé-Amat, J., Lorz, C., Ponz, F. 2024. A Multifunctionalized Potyvirus-Derived Nanoparticle That Targets and Internalizes into Cancer Cells. International Journal of Molecular Sciences 25, 4327. DOI: 10.3390/ijms25084327

Phelps, A., Pazos-Castro, D., Urselli, F., Grydziuszko, E., Mann-Delany, O., Fang, A., Walker, T.D., Guruge, R.T., Tome-Amat, J., Diaz-Perales, A., Waserman, S., Boonyaratanakornkit, J., Jordana, M., Taylor, J.J., Koenig, J.F.E. 2024. Production and use of antigen tetramers to study antigen-specific B cells. Nature Protocols 1–25. DOI: 10.1038/s41596-023-00930-8

Aguilella, M., Garciadeblás, B., Fernández Pacios, L., Benito, B. 2024. Phylogenetic and Structure-Function Analyses of ENA ATPases: A Case Study of the ENA1 Protein from the Fungus Neurospora crassa. International Journal of Molecular Sciences 25, 514. DOI: 10.3390/ijms25010514

Pacios, L.F., Sánchez, F., Ponz, F. 2024. Intrinsic disorder in the dynamic evolution of structure, stability, and flexibility of potyviral VLP assemblies: A computational study. International Journal of Biological Macromolecules 127798. DOI: 10.1016/j.ijbiomac.2023.127798

Rodríguez, S., García-García, A., Garcia-Calvo, E., Esteban, V., Pastor-Vargas, C., Díaz-Perales, A., García, T., Martín, R. 2023. Generation of an Ovomucoid-Immune scFv Library for the Development of Novel Immunoassays in Hen’s Egg Detection. Foods 12, 3831. DOI: 10.3390/foods12203831

Tome-Amat, J., Gonzalez-Klein, Z., Pazos-Castro, D., Resuela-Gonzalez, J.L., Vilchez-Pinto, G., Berreiros-Hortala, H., Palacio-Garcia, L., Fernandez-Bravo, S., Esteban, V., Diaz-Perales, A., Garrido-Arandia, M. 2023. Plant lipid transfer proteins’ ligand enhances allergic sensitization to non-related proteins in murine models. Allergy. DOI: 10.1111/all.15898

Mínguez-Toral, M., Pacios, L.F., Sánchez, F., Ponz, F. 2023. Structural intrinsic disorder in a functionalized potyviral coat protein as a main viability determinant of its assembled nanoparticles. International Journal of Biological Macromolecules 236, 123958. DOI: 10.1016/j.ijbiomac.2023.123958

Martín-Dacal, M., Fernández-Calvo, P., Jiménez-Sandoval, P., López, G., Garrido-Arandía, M., Rebaque, D., del Hierro, I., Berlanga, D.J., Torres, M.Á., Kumar, V., Mélida, H., Pacios, L.F., Santiago, J., Molina, A. 2022. Arabidopsis immune responses triggered by cellulose and mixed-linked glucan oligosaccharides require a group of Leucine-Rich Repeat-Malectin Receptor Kinases. The Plant Journal. DOI: 10.1111/tpj.16088

Dijk, W., Villa, C., Benedé, S., Vassilopoulou, E., Mafra, I., Garrido-Arandia, M., Martínez Blanco, M., Bouchaud, G., Hoppenbrouwers, T., Bavaro, S.L., Giblin, L., Knipping, K., Castro, A.M., Delgado, S., Costa, J., Bastiaan-Net, S. 2022. Critical features of an in vitro intestinal absorption model to study the first key aspects underlying food allergen sensitization. Comprehensive Reviews in Food Science and Food Safety. DOI: 10.1111/1541-4337.13097

Meile, L., Garrido-Arandia, M., Bernasconi, Z., Peter, J., Schneller, A., Bernasconi, A., Alassimone, J., McDonald, B.A., Sánchez-Vallet, A. 2022. Natural variation in Avr3D1 from Zymoseptoria sp. contributes to quantitative gene-for-gene resistance and to host specificity. New Phytologist. DOI: 10.1111/nph.18690

Regner, A., Szepannek, N., Wiederstein, M., Fakhimahmadi, A., Pacios, L.F., Blokhuis, B.R., Redegeld, F.A., Hofstetter, G., Dvorak, Z., Jensen-Jarolim, E., Hufnagl, K., Roth-Walter, F. 2023. Binding to Iron Quercetin Complexes Increases the Antioxidant Capacity of the Major Birch Pollen Allergen Bet v 1 and Reduces Its Allergenicity. Antioxidants 12, 42. DOI: 10.3390/antiox12010042

Pazos-Castro, D., Margain, C., Gonzalez-Klein, Z., Yuste-Calvo, C., Garrido-Arandia, M., Zurita, L., Esteban, V., Tome-Amat, J., Diaz-Perales, A., Ponz, F. 2022. Suitability of potyviral recombinant virus-like particles bearing a complete food allergen for immunotherapy vaccines. Frontiers in Immunology 13. DOI: 10.3389/fimmu.2022.986823

Murillo, A.M.M., Valle, L.G., Ramírez, Y., Sánchez, M.J., Santamaría, B., Molina-Roldan, E., Ortega-Madueño, I., Urcelay, E., Tramarin, L., Herreros, P., Díaz-Perales, A., Garrido-Arandia, M., Tome-Amat, J., Hernández-Ramírez, G., Espinosa, R.L., Laguna, M.F., Holgado, M. 2022. Integration of Multiple Interferometers in Highly Multiplexed Diagnostic KITs to Evaluate Several Biomarkers of COVID-19 in Serum. Biosensors 12, 671. DOI: 10.3390/bios12090671

Diem, L., Neuherz, B., Rohrhofer, J., Koidl, L., Asero, R., Brockow, K., Diaz Perales, A., Faber, M., Gebhardt, J., Torres, M., Jensen-Jarolim, E., Zehetmayer, S., Untersmayr, E. n.d. Real-life evaluation of molecular multiplex IgE test methods in the diagnosis of pollen associated food allergy. Allergy n/a. DOI: 10.1111/all.15329

Missaoui, K., Gonzalez-Klein, Z., Jemli, S., Garrido-Arandia, M., Diaz-Perales, A., Tome-Amat, J., Brini, F. 2022. Identification and molecular characterization of a novel non-specific lipid transfer protein (TdLTP2) from durum wheat. PLOS ONE 17, e0266971. DOI: 10.1371/journal.pone.0266971

Pali-Schöll, I., Bianchini, R., Afify, S.M., Hofstetter, G., Winkler, S., Ahlers, S., Altemeier, T., Mayerhofer, H., Hufnagl, K., Korath, A.D.J., Pranger, C., Widhalm, R., Hann, S., Wittek, T., Kasper-Giebl, A., Pacios, L.F., Roth-Walter, F., Vercelli, D., von Mutius, E., Jensen-Jarolim, E. 2022. Secretory protein beta-lactoglobulin in cattle stable dust may contribute to the allergy-protective farm effect. Clinical and Translational Allergy 12, e12125. DOI: 10.1002/clt2.12125

Pazos-Castro, D., Gonzalez-Klein, Z., Montalvo, A.Y., Hernandez-Ramirez, G., Romero-Sahagun, A., Esteban, V., Garrido-Arandia, M., Tome-Amat, J., Diaz-Perales, A. 2022. NLRP3 priming due to skin damage precedes LTP allergic sensitization in a mouse model. Scientific Reports 12, 3329. DOI: 10.1038/s41598-022-07421-y

Missaoui, K., Gonzalez-Klein, Z., Pazos-Castro, D., Hernandez-Ramirez, G., Garrido-Arandia, M., Brini, F., Diaz-Perales, A., Tome-Amat, J. 2022. Plant non-specific lipid transfer proteins: An overview. Plant Physiology and Biochemistry 171, 115–127. DOI: 10.1016/j.plaphy.2021.12.026

Hernandez-Ramirez, G., Barber, D., Tome-Amat, J., Garrido-Arandia, M., Diaz-Perales, A. 2021. Alternaria as an Inducer of Allergic Sensitization. Journal of Fungi 7, 838. DOI: 10.3390/jof7100838

Phillips, A.H., Hernandez, J.A., Payá-Tormo, L., Burén, S., Cuevas-Zuviría, B., Pacios, L.F., Pelton, J.G., Wemmer, D.E., Rubio, L.M. 2021. Environment and coordination of FeMo–co in the nitrogenase metallochaperone NafY. RSC Chemical Biology. DOI: 10.1039/D1CB00086A

Murillo, A.M.M., Tomé-Amat, J., Ramírez, Y., Garrido-Arandia, M., Valle, L.G., Hernández-Ramírez, G., Tramarin, L., Herreros, P., Santamaría, B., Díaz-Perales, A., Holgado, M. 2021. Developing an optical interferometric detection method based biosensor for the detection of detecting specific SARS-CoV-2 immunoglobulins in serum and saliva, and their corresponding ELISA correlation. Sensors and Actuators B: Chemical 130394. DOI: 10.1016/j.snb.2021.130394

Skypala, I.J., Asero, R., Barber, D., Cecchi, L., Diaz-Perales, A., Hoffmann-Sommergruber, K., Pastorello, E.A., Swoboda, I., Bartra, J., Ebo, D.G., Faber, M.A., Fernández-Rivas, M., Gomez, F., Konstantinopoulos, A.P., Luengo, O., Ree, R. van, Scala, E., Till, S.J. 2021. Non-specific lipid-transfer proteins: Allergen structure and function, cross-reactivity, sensitization, and epidemiology. Clinical and Translational Allergy 11, e12010. DOI: 10.1002/clt2.12010

Gonzalez-Klein, Z., Cuevas-Zuviria, B., Wangorsch, A., Hernandez-Ramirez, G., Pazos-Castro, D., Oeo-Santos, C., Romero-Sahagun, A., Pacios, L.F., Tome-Amat, J., Scheurer, S., Diaz-Perales, A., Garrido-Arandia, M. 2021. The key to the allergenicity of lipid transfer protein (LTP) ligands: A structural characterization. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids 1866, 158928. DOI: 10.1016/j.bbalip.2021.158928

Cuevas-Zuviría, B., Pacios, L.F. 2021. Machine Learning of Analytical Electron Density in Large Molecules Through Message-Passing. Journal of Chemical Information and Modeling. DOI: 10.1021/acs.jcim.1c00227

Skypala, I.J., Bartra, J., Ebo, D.G., Faber, M.A., Fernández‐Rivas, M., Gomez, F., Luengo, O., Till, S.J., Asero, R., Barber, D., Cecchi, L., Diaz-Perales, A., Hoffmann‐Sommergruber, K., Pastorello, E.A., Swoboda, I., Konstantinopoulos, A.P., Ree, R. van, Scala, E. 2021. The diagnosis and management of allergic reactions in patients sensitized to non-specific Lipid Transfer Proteins. Allergy. DOI:

Costa, J., Villa, C., Verhoeckx, K., Cirkovic-Velickovic, T., Schrama, D., Roncada, P., Rodrigues, P.M., Piras, C., Martín-Pedraza, L., Monaci, L., Molina, E., Mazzucchelli, G., Mafra, I., Lupi, R., Lozano-Ojalvo, D., Larré, C., Klueber, J., Gelencser, E., Bueno-Diaz, C., Diaz-Perales, A., Benedé, S., Bavaro, S.L., Kuehn, A., Hoffmann-Sommergruber, K., Holzhauser, T. 2021. Are Physicochemical Properties Shaping the Allergenic Potency of Animal Allergens?. Clinical Reviews in Allergy & Immunology. DOI: 10.1007/s12016-020-08826-1

Espinosa, R.L., Garrido-Arandia, M., Romero-Sahagun, A., Herreros, P., Tramarin, L., Laguna, M.F., Díaz-Perales, A., Holgado, M. 2020. A new optical interferometric-based in vitro detection system for the specific IgE detection in serum of the main peach allergen. Biosensors and Bioelectronics 169, 112641. DOI: 10.1016/j.bios.2020.112641

Costa, J., Bavaro, S.L., Benedé, S., Diaz-Perales, A., Bueno-Diaz, C., Gelencser, E., Klueber, J., Larré, C., Lozano-Ojalvo, D., Lupi, R., Mafra, I., Mazzucchelli, G., Molina, E., Monaci, L., Martín-Pedraza, L., Piras, C., Rodrigues, P.M., Roncada, P., Schrama, D., Cirkovic-Velickovic, T., Verhoeckx, K., Villa, C., Kuehn, A., Hoffmann-Sommergruber, K., Holzhauser, T. 2020. Are Physicochemical Properties Shaping the Allergenic Potency of Plant Allergens?. Clinical Reviews in Allergy & Immunology. DOI: 10.1007/s12016-020-08810-9

Cuevas-Zuviría, B., Pacios, L.F. 2020. Analytical Model of Electron Density and Its Machine Learning Inference. Journal of Chemical Information and Modeling 60, 3831–3842. DOI: 10.1021/acs.jcim.0c00197

Mínguez-Toral, M., Cuevas-Zuviría, B., Garrido-Arandia, M., Pacios, L.F. 2020. A computational structural study on the DNA-protecting role of the tardigrade-unique Dsup protein. Scientific Reports 10, 13424. DOI: 10.1038/s41598-020-70431-1

López‐González, S., Navarro, J.A., Pacios, L.F., Sardaru, P., Pallás, V., Sánchez, F., Ponz, F. 2020. Association between flower stalk elongation, an Arabidopsis developmental trait, and the subcellular location and movement dynamics of the nonstructural protein P3 of Turnip mosaic virus. Molecular Plant Pathology. DOI: 10.1111/mpp.12976

Roth-Walter, F., Afify, S.M., Pacios, L.F., Blokhuis, B.R., Redegeld, F., Regner, A., Petje, L.-M., Fiocchi, A., Untersmayr, E., Dvorak, Z., Hufnagl, K., Pali-Schöll, I., Jensen-Jarolim, E. 2020. Cow’s milk protein β-lactoglobulin confers resilience against allergy by targeting complexed iron into immune cells. Journal of Allergy and Clinical Immunology. DOI: 10.1016/j.jaci.2020.05.023

Hernández‐Ramírez, G., Pazos‐Castro, D., Torrijos, E.G., Montalvo, A.Y., Romero‐Sahagun, A., González‐Klein, Z., Jimeno‐Nogales, L., Escribese, M.M., Ortega, A.E., Nuñez‐Borque, E., Orvay, L.B., Esteban, V., Brito, F.F., Barber, D., Tome‐Amat, J., Garrido‐Arandia, M., Díaz‐Perales, A. 2020. Group 1 allergens, transported by mold spores, induce asthma exacerbation in a mouse model. Allergy. DOI: 10.1111/all.14347

Cuevas-Zuviría, B., Mínguez-Toral, M., Díaz-Perales, A., Garrido-Arandia, M., Pacios, L.F. 2020. Structural Dynamics of the Lipid Antigen-Binding Site of CD1d Protein. Biomolecules 10, 532. DOI: 10.3390/biom10040532

Cuevas-Zuviría, B., Mínguez-Toral, M., Díaz-Perales, A., Garrido-Arandia, M., Pacios, L.F. 2020. Dynamic plasticity of the lipid antigen-binding site of CD1d is crucially favoured by acidic pH and helper proteins. Scientific Reports 10, 5714. DOI: 10.1038/s41598-020-62833-y

Blanca, M., Puche, L.V., Garrido-Arandia, M., Martin-Pedraza, L., Sahagún, A.R., López-Sánchez, J.D., Galán, C., Marin, A., Villaba, M., Díaz-Perales, A., Somoza, M.L. 2020. Pru p 9, a new allergen eliciting respiratory symptoms in subjects sensitized to peach tree pollen. PLOS ONE 15, e0230010. DOI: 10.1371/journal.pone.0230010

Orozco-Navarrete, B., Kaczmarska, Z., Dupeux, F., Garrido-Arandia, M., Pott, D., Díaz-Perales, A., Casañal, A., Márquez, J.A., Valpuesta, V., Merchante, C. 2019. Structural Bases for the Allergenicity of Fra a 1.02 in Strawberry Fruits. Journal of Agricultural and Food Chemistry. DOI: 10.1021/acs.jafc.9b05714

Oeo‐Santos, C., Navas, A., Benedé, S., Ruíz‐León, B., Díaz‐Perales, A., Vogel, L., Moreno‐Aguilar, C., Jurado, A., Villalba, M., Barderas, R. 2019. New insights into the sensitization to non-related nsLTPs from pollen and food: new role of the allergen Ole e 7. Allergy. DOI: 10.1111/all.14086

Victorio Puche, L., Somoza, M.L., López-Sánchez, J.D., Garrido-Arandia, M., Díaz-Perales, A., Blanca, M. 2019. Peach Tree Pollen and Prunus persica 9 Sensitisation and Allergy in Children and Adolescents. International Archives of Allergy and Immunology 180, 212–220. DOI: 10.1159/000502352

Delgado, R., Abad-Guamán, R., Nicodemus, N., Diaz-Perales, A., García, J., Carabaño, R., Menoyo, D. 2019. Effect of pre- and post-weaning dietary supplementation with arginine and glutamine on rabbit performance and intestinal health. BMC Veterinary Research 15, 199. DOI: 10.1186/s12917-019-1945-2

Garrido‐Arandia, M., Tome‐Amat, J., Pazos‐Castro, D., Esteban, V., Escribese, M.M., Hernández‐Ramírez, G., Yuste, A.M., Barber, D., Pacios, L.F., Díaz‐Perales, A. 2019. Interaction of Alt a 1 with SLC22A17 in the airway mucosa. Allergy. DOI: 10.1111/all.13877

Somoza, M.L., Garrido‐Arandia, M., Victorio, L., López‐Sánchez, J.D., Blanca‐López, N., Haroun‐Diaz, E., Sahagún, A.R., Díaz‐Perales, A., Canto, G., Blanca, M. 2019. Peach tree pollen and Pru p 9 may induce rhinoconjunctivitis and asthma in children. Pediatric Allergy and Immunology. DOI: 10.1111/pai.13067

Yuste-Calvo, C., González-Gamboa, I., Pacios, L.F., Sánchez, F., Ponz, F. 2019. Structure-Based Multifunctionalization of Flexuous Elongated Viral Nanoparticles. ACS Omega 4, 5019–5028. DOI: 10.1021/acsomega.8b02760

Cuevas-Zuviría, B., Garrido-Arandia, M., Díaz-Perales, A., Pacios, L.F. 2019. Energy Landscapes of Ligand Motion Inside the Tunnel-Like Cavity of Lipid Transfer Proteins: The Case of the Pru p 3 Allergen. International Journal of Molecular Sciences 20, 1432. DOI: 10.3390/ijms20061432

Lozano-Ojalvo, D., Benedé, S., Antunes, C.M., Bavaro, S.L., Bouchaud, G., Costa, A., Denery-Papini, S., Díaz-Perales, A., Garrido-Arandia, M., Gavrovic-Jankulovic, M., Hayen, S., Martínez-Blanco, M., Molina, E., Monaci, L., Pieters, R.H.H., Villemin, C., Wichers, H.J., Wróblewska, B., Willemsen, L.E.M., Roggen, E.L., van Bilsen, J.H.M. 2019. Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies. Trends in Food Science & Technology 85, 307–319. DOI: 10.1016/j.tifs.2019.01.014

Albareda, M., Pacios, L.F., Palacios, J.M. 2019. Computational analyses, molecular dynamics, and mutagenesis studies of unprocessed form of [NiFe] hydrogenase reveal the role of disorder for efficient enzyme maturation. Biochimica et Biophysica Acta (BBA) - Bioenergetics 1860, 325–340. DOI: 10.1016/j.bbabio.2019.01.001

Rodriguez, M.J., Palomares, F., Bogas, G., Torres, M.J., Diaz‐Perales, A., Rojo, J., Plaza‐Seron, M. del C., Rodriguez‐Nogales, A., Orengo, C., Mayorga, C., Perkins, J.R. 2018. Transcriptional profiling of dendritic cells in a mouse model of food-antigen induced anaphylaxis reveals the upregulation of multiple immune-related pathways. Molecular Nutrition & Food Research 1800759. DOI: 10.1002/mnfr.201800759

Mazzucchelli, G; Holzhauser, T; Cirkovic Velickovic, T; Diaz-Perales, A; Molina, E; Roncada, P; Rodrigues, P; Verhoeckx, K; Hoffmann-Sommergruber, K. 2018. "Current (food) allergenic risk assessment: Is it fit for novel foods? Status Quo and identification of gaps". Molecular Nutrition and Food Research. DOI: 10.1002/mnfr.201700278".

Safi, H; Wangorsch, A; Lidholm, J; Brini, F; Spiric, J; Rihs, H-P; Vieths, S; Armentia, A; Farioli, L; Diaz-Perales, A; Pastorello, EA; Scheurer, S. "Identification and molecular characterization of allergenic nsLTP from durum wheat (Triticum turgidum)". Clinical & Experimental Allergy. DOI: 10.1111/cea.13271".

Gómez-Gómez, L; Pacios, LF; Diaz-Perales, A; Garrido-Arandia, M; Argandoña, J; Rubio-Moraga, Á; Ahrazem, O. 2018. "Expression and interaction analysis among saffron ALDHs and crocetin dialdehyde". International Journal of Molecular Sciences. DOI: 10.3390/ijms19051409".

Palomares, F; Gomez, F; Bogas, G; Campo, P; Perkins, JR; Diaz-Perales, A; Rodriguez, MJ; Prieto, A; Barber, D; Torres, MJ; Mayorga, C. 2018. "Immunological changes induced in peach allergy patients with systemic reactions by Pru p 3 sublingual immunotherapy". Molecular Nutrition & Food Research. DOI: 10.1002/mnfr.201700669".

Garrido-Arandia, M; Cuevas-Zuviria, B; Diaz-Perales, A; Pacios, LF. 2018. "A comparative study of human saposins". Molecules. DOI: 10.3390/molecules23020422".

Hufnagl, K; Ghosh, D; Wagner, S; Fiocchi, A; Dahdah, L; Bianchini, R; Braun, N; Steinborn, R; Hofer, M; Blaschitz, M; Roth, GA; Hofstetter, G; Roth-Walter, F; Pacios, LF; Jensen-Jarolim, E. 2018. "Retinoic acid prevents immunogenicity of milk lipocalin Bos d 5 through binding to its immunodominant T-cell epitope". Scientific Reports. DOI: 10.1038/s41598-018-19883-0".

Roth-Walter, F; Pacios, LF; Bianchini, R; Jensen-Jarolim, E. 2017. "Linking iron-deficiency with allergy: role of molecular allergens and the microbiome". Metallomics. DOI: 10.1039/c7mt00241f".

Mélida, H; Sopeña-Torres, S; Bacete, L; Garrido-Arandia, M; Jordá, L; López, G; Muñoz, A; Pacios, LF; Molina, A. 2017. "Non-branched β-1,3-glucan oligosaccharides trigger immune responses in Arabidopsis". Plant Journal. DOI: 10.1111/tpj.13755".

Tordesillas, L; Cubells-Baeza, N; Gómez-Casado, C; Berin, C; Esteban, V; Barcik, W; O'Mahony, L; Ramirez, C; Pacios, LF; Garrido-Arandia, M; Díaz-Perales, A. 2017. "Mechanisms underlying induction of allergic sensitization by Pru p 3". Clinical & Experimental Allergy. DOI: 10.1111/cea.12962".

Mas, S; Oeo-Santos, C; Cuesta-Herranz, J; Diaz-Perales, A; Colas, C; Fernandez, J; Barber, D; Rodriguez, R; de Los Rios, V; Barderas, R; Villalba, M. 2017. "A relevant IgE-reactive 28kDa protein identified from Salsola kali pollen extract by proteomics is a natural degradation product of an integral 47kDa polygalaturonase". Biochimica et Biophysica Acta. DOI: 10.1016/j.bbapap.2017.05.007".

Benedé, S; Garrido-Arandia, M; Martín-Pedraza, L; Bueno, C; Díaz-Perales, A; Villalba, M. 2017. "Multifactorial modulation of food-induced anaphylaxis". Frontiers in Immunology. DOI: 10.3389/fimmu.2017.00552".

Cubells-Baeza, N; Gómez-Casado, C; Tordesillas, L; Ramírez-Castillejo, C; Garrido-Arandia, M; González-Melendi, P; Herrero, M; Pacios, LF; Díaz-Perales, A. 2017. "Identification of the ligand of Pru p 3, a peach LTP". Plant Molecular Biology. DOI: 10.1007/s11103-017-0590-z".

Gomez, F; Bogas, G; Gonzalez, M; Campo, P; Salas, M; Diaz-Perales, A; Rodriguez, MJ; Prieto, A; Barber, D; Blanca, M; Torres, MJ; Mayorga, C. 2017. "The clinical and immunological effects of Pru p 3 SLIT on peach and peanut allergy in patients with systemic reactions". Clinical & Experimental Allergy. DOI: 10.1111/cea.12901".

Lavilla, M; Orcajo, J; Díaz-Perales, A; Gamboa, P. 2016. "Examining the effect of High Pressure Processing on the allergenic potential of the major allergen in peach (Pru p 3)". Innovative Food Science & Emerging Technologies. DOI: 10.1016/j.ifset.2016.06.021".

Gómez-Casado, C; Díaz-Perales, A. 2016. "Allergen-associated immunomodulators: modifying allergy outcome". Archivum Immunologiae et Therapiae Experimentalis. DOI: 10.1007/s00005-016-0401-2".

Cubells-Baeza, N; Verhoeckx, KCM; Larre, C; Denery-Papini, S; Gavrovic-Jankulovic, M; Diaz Perales, A. 2016. "Applicability of epithelial models in protein permeability/transport studies and food allergy". Drug Discovery Today: Disease Models. DOI: 10.1016/j.ddmod.2016.08.002".

Garrido-Arandia, M; Silva-Navas, J; Ramírez-Castillejo, C; Cubells-Baeza, N; Gómez-Casado, C; Barber, D; Pozo, JC; Melendi, PG; Pacios, LF; Díaz-Perales, A. 2016. "Characterisation of a flavonoid ligand of the fungal protein Alt a 1". Scientific Reports. DOI: 10.1038/srep33468".

Poulicard, N; Pacios, LF; Gallois, J-L; Piñero, D; García-Arenal, F. 2016. "Human management of a wild plant modulates the evolutionary dynamics of a gene determining recessive resistance to virus infection". PLoS Genetics. DOI: 10.1371/journal.pgen.1006214".

Pastor-Vargas, C; Maroto, AS; Díaz-Perales, A; Villalba, M; Esteban, V; Ruiz-Ramos, M; Rodriguez de Alba, M; Vivanco, F; Cuesta-Herranz, J. 2016. "Detection of major food allergens in amniotic fluid: initial allergenic encounter during pregnancy". Pediatric Allergy and Immunology. DOI: 10.1111/pai.12608".

Garrido-Arandia, M; Bretones, J; Gómez-Casado, C; Cubells, N; Díaz-Perales, A; Pacios, LF. 2016. "Computational study of pH-dependent oligomerization and ligand binding in Alt a 1, a highly allergenic protein with a unique fold". Journal of Computer-Aided Molecular Design. DOI: 10.1007/s10822-016-9911-6".

Pascal, M; Muñoz-Cano, R; Milà, J; Sanz, ML; Diaz-Perales, A; Sánchez-López, J; García-Moral, A; Juan, M; Valero, A; Yagüe, J; Picado, C; Bartra, J. 2016. "Nonsteroidal anti-inflammatory drugs enhance IgE-mediated activation of human basophils in patients with food anaphylaxis dependent and independent of nonsteroidal anti-inflammatory drugs". Clinical & Experimental Allergy. DOI: 10.1111/cea.12735".

Goikoetxea, M; D’Amelio, C; Martínez-Aranguren, R; Gamboa, P; Garcia, B; Gómez, F; Fernández, J; Bartra, J; Parra, A; Alvarado, M; Alonso, M; González, E; Terrados, S; Moya, C; Blanca, N; Feo-Brito, F; Villalba, M; Díaz-Perales, A; Sanz, M. 2016. "Is Microarray Analysis Really Useful and Sufficient to Diagnose Nut Allergy in the Mediterranean Area?". Journal of Investigational Allergology and Clinical Immunology. DOI: 10.18176/jiaci.0005".

Martín-Muñoz, MF; Rivero, D; Díaz Perales, A; Polanco, I; Quince, S. 2016. "Wheat allergy in celiac children". Pediatric Allergy and Immunology. DOI: 10.1111/pai.12487".

Jensen-Jarolim, E; Pacios, LF; Bianchini, R; Hofstetter, G; Roth-Walter, F. 2015. "Structural similarities of human & mammalian lipocalins, and their function in innate immunity and allergy". Allergy. DOI: 10.1111/all.12797".

Mas, S; Boissy, P; Monsalve, RI; Cuesta-Herranz, J; Díaz-Perales, A; Fernández, J; Colás, C; Rodríguez, R; Barderas, R; Villalba, M. 2015. "A recombinant Sal k 1 isoform as an alternative to the polymorphic allergen from Salsola kali pollen for allergy diagnosis". International Archives of Allergy and Immunology. DOI: 10.1159/000434680".

Albareda, M.; Pacios, L.F.; Manyani, H.; Rey, L.; Brito, B.; Imperial, J.; Ruiz-Argueso, T.; Palacios, J.M. 2014. "Maturation of Rhizobium leguminosarum hydrogenase in the presence of oxygen requires the interaction of the chaperone HypC and the scaffolding protein HupK". Journal of Biological Chemistry 289, 21217-21229. DOI: 10.1074/jbc.M114.577403".

Fraile, A; Hily, J-M; Pagán, I; Pacios, LF; García-Arenal, F. 2014. "Host resistance selects for traits unrelated to resistance-breaking that affect fitness in a plant virus". Molecular Biology and Evolution. DOI: 10.1093/molbev/msu045".

Gómez-Casado, C; Roth-Walter, F; Jensen-Jarolim, E; Díaz-Perales, A; Pacios, LF. 2013. "Modeling iron-catecholates binding to NGAL protein". Journal of Molecular Graphics & Modelling. DOI:".