New types of viral nanoparticles derived from elongated virus have been developed in the CBGP group ‘Plant Virus Biotehcnology’. These new nanoparticles allow functionalization with molecules hitherto unsuccessful.
In recent years, plant viral nanoparticles (VNP) have become an attractive platform for the development of new nanotools due to their safety, low-cost production and easy functionalization. Turnip mosaic virus (TuMV) is an example of a plant-derived NPV used as a nanobiotechnological platform based on its functionalization with different molecules. In this article, we demonstrate the successful application of SpyTag/SpyCatcher technology as an alternative for the functionalization of TuMV VLPs with peptides and proteins. This technology, a mixed chemogenetic approach to viral nanoparticle functionalization, has allowed us to create nanotools that could not be obtained before by alternative methods such as chemical conjugation and gene fusion.
Truchado, D.A., Rincón, S., Zurita, L., Sánchez, F., Ponz, F. 2023. Isopeptide Bonding In Planta Allows Functionalization of Elongated Flexuous Proteinaceous Viral Nanoparticles, including Non-Viable Constructs by Other Means. Viruses 15, 375. DOI: 10.3390/v15020375