A research project led by Dra. María A. Ayllón from CBGP, in collaboration with Dra. Livia Donaire, has shown that gene silencing in Botrytis acts as mechanism of defense against the infection by different mycoviruses.
The gene silencing is a mechanism that controls several developmental processes in plants, animals and fungi, and is also a potent antiviral defense mechanism. The process begins when the virus enters the host cell with the production of molecules with double-stranded RNA features derived from the virus that are recognized by a specific type of RNase III called Dicer and sliced into short RNA duplexes, between 21 and 24 nucleotides, that are called viral small RNAs. They act by binding to other plant proteins called Argonauta and direct them towards complementary viral RNA molecules which will be degraded by the endonuclease activity of these proteins. The knowledge of the characteristics of the populations of small RNAs formed during viral infections can help us to understand this important antiviral defense mechanism and use it in control strategies.
In this work we have done a comprehensive study of the characteristics, abundance and diversity of populations of small RNAs derived from different species of mycoviruses infecting isolates of two species of the fungus