Single-cell disection of Lateral Root ontogeny

Plants can generate new organs throughout their life. We dissect through Single-cell RNA-seq how lateral roots (the postembric organs that form the bulk of the root systems) are generated from precursor cells, thus initiating tissue formation through functional specialization and progressive acquisition of molecular identity fingerprints.


We describe a method for capturing design information of cellular communities. In a similar way we design a genetic construct in-silico by writing and annotating the DNA sequence, here we use the Synthetic Biology Open Language (SBOL) to come up with a procedure to write and annotate multicellular systems. This enables sharing designs in a standard format, thus increasing reproducibility.


Original Paper:

Brown, B., Bartley, B., Beal, J., Bird, J.E., Goñi-Moreno, Á., McLaughlin, J.A., Mısırlı, G., Roehner, N., Skelton, D.J., Poh, C.L., Ofiteru, I.D., James, K., Wipat, A. 2020. Capturing Multicellular System Designs Using Synthetic Biology Open Language (SBOL). ACS synthetic biology 9, 2410–2417. DOI: 10.1021/acssynbio.0c00176