Jasmonate-derivatives modulate plant defences against phytophagous mites

To deepen into the jasmonic acid (JA) catabolism and its impact on plant responses to spider mite (Tetranychus urtciae) infestation, we selected the Arabidopsis JAO2 gene as a key element involved in the first step of the JA-catabolic route. JAO2 is responsible for the hydroxylation of JA into 12-OH-JA, contributes to attenuate JA and JA-Ile content and consequently, determines the formation of other JA-catabolites. JAO2 was up-regulated in Arabidopsis by mite infestation. Mites also induced JA-derivative accumulation in plants. In jao2 mutant lines, and in the triple mutant jaoT (jao2 -1, jao3 -1, jao4 -2), mite feeding produced less leaf damage, minor callose deposition and lower mite fecundity rates than in Col-0 plants. The impairment of JA oxidation in jao2 lines not only diminished the 12-OH-JA levels but turned off further sulfation as shown the significant reduction of 12- HSO4-JA form. Thus, JAO2 acts as a negative modulator of defences to spider mites mediated by changes in the generation of JA catabolic molecules, and the consequent production of defensive metabolites such as glucosinolates or camalexin.


Original Paper:

Rosa-Diaz, I., Santamaria, M.E., Acien, J.M., Diaz, I. 2023. Jasmonic acid catabolism in Arabidopsis defence against mites. Plant Science 334, 111784. DOI: 10.1016/j.plantsci.2023.111784