The peach-potato aphid Myzus persicae (Sulzer) and the black bean aphid Aphis fabae Scopoli are polyphagous and Cosmopolitan hemipterans, therefore they can infest grapevines in all areas of cultivation. The present study revealed that all studied species of grapevines, V. amurensis, V. riparia and V. vinifera are relatively resistant to A. fabae and M. persicae. These grape-vines showed features related to the activity of antixenosis factors at the level of mesophyll and phloem tissues, which was manifested in difficulties in tissue penetration by aphids, little success in reaching the feeding phase and its short duration. Vitis vinífera was the least susceptible grapevine species to A. fabae. No differences in the susceptibility among grapevines occurred against M. persicae. However, considering the higher success rate in reaching phloem phase and longer duration of probing, it can be inferred that M. persicae poses greater threat to the studied grapevines than A. fabae. The longer duration of probing increases the risk of virus transmission. Differences in anatomical structure of the leaves did not relate with aphid probing behavior. However, flavonoids and stilbenoids may have caused the reduction of probing in phloem tissues. Therefore, the unambiguous determination of the direct influence of individual flavonoids and stilbenoids and particularly, piceid, on the behavior of M. persicae and A. fabae requires further, more detailed studies involving the application of artificial diets.