In recent years, plant viral nanoparticles (VNP) have become an attractive platform for the development of new nanotools due to their safety, low-cost production and easy functionalization. Turnip mosaic virus (TuMV) is an example of a plant-derived NPV used as a nanobiotechnological platform based on its functionalization with different molecules. In this article, we demonstrate the successful application of SpyTag/SpyCatcher technology as an alternative for the functionalization of TuMV VLPs with peptides and proteins. This technology, a mixed chemogenetic approach to viral nanoparticle functionalization, has allowed us to create nanotools that could not be obtained before by alternative methods such as chemical conjugation and gene fusion.