REGULATORY NETWORKS IN THE SEED: INTEGRATION OF DEVELOPMENT, METABOLISM AND ENVIRONMENTAL CONDITIONS

Group leader: Jesús Vicente Carbajosa - Professor
This email address is being protected from spambots. You need JavaScript enabled to view it.  910679182 (Office 130 )   910679131 (Lab 132 )

 

Personnel:

 

Research interests

 

Our main research interest is transcriptional control of gene expression, primarily in the field of seed development. In this framework our group identified and characterized several transcription factors participating in the regulation of gene expression programmes associated to the seed maturation and germination phases. In addition, we are interested in the study of transcription factors affecting metabolic adjustment to carbon/nitrogen partitioning and stress responses.

 

We are investigating regulatory networks, based mainly on transcription factors (TFs) of the bZIP, DOF, MYB and B3 classes. Our group is currently performing transcriptomic analyses of Arabidopsis transgenic plants affected in the expression of transcriptions factors controlling key aspects of seed development and the validation of potentially regulated genes in transient expression assays. Other techniques relevant to our research include the study of protein-DNA and protein-protein interactions, in situ RNA hybridisation and comparative bioinformatic analyses of TF families, mostly in Arabidopsis and cereal species.

 

Function and Biotechnological potential of seed Transcription Factors

 

Transcription factors (TFs) are regulatory proteins that have a key role in evolution and have also a great biotechnological potential. They are crucial in regulating gene expression in a combinatorial way and they have been important in domestication and plant breeding; some examples are Teosinte Branched 1 that affects plant architecture (branching), the Green Revolution Genes (GRAS) responsible of the semi-dwarf cultivars of presently grown cereals, the DOF1 gene that improve nitrogen assimilation and growth under low nitrogen conditions, etc. The identification of key regulatory transcription factors (TFs) involved in the maturation and germination of seeds from crops (barley) and from model plants (Arabidopsis thaliana and Brachypodium distachyon) has been an aim of our group in the past years and a number of TF genes belonging to several TF families, such as bZIP, DOF and MYB have been demonstrated to be important in both processes. However, it is still unclear to what extent these TF genes are regulated in monocot- and dicotyledonous seeds. The AFL sub-family of B3 TFs (VP1/ABI3, FUS3, LEC2) has been recently studied and its origin, through the course of evolution, traced back to non-vascular plants such as Physcomitrella patens and its function explored taking into account expression patterns and interactions with other TFs. The first member of this family to be described was the maize Viviparous-1 (Vp-1) as a central player of Pre-Harvest Sprouting and as a regulator of the C1 gene encoding a MYB-like TF regulator of the anthocyanin biosynthesis pathway. Its ortholog from barley (HvVP1) has been demonstrated to play essential roles in the regulation of genes upon maturation and germination through interaction with GAMYB, BPBF and BLZ2. HvFUS3 from barley unveils a common transcriptional regulation of seed specific genes between cereals and Arabidopsis (AtFUS3).


 

 

During germination of Arabidopsis thaliana seeds, the AtMAN7 gene that encodes an endo-β-mananase (MAN; EC. 3.2.1.78) is critical for hydrolyzing the seed covering layers that are rich in mannans. The bioinformatic analysis of the promoter sequences of orthologous genes of AtMAN7 in several species of the Brassicaceae family (phylogenomics) discovered highly conserved motives. These conserved motives were used as baits in a yeast 1-hybrid screening of an arrayed yeast library of circa 1,200 TFs from A. thaliana (Castrillo et al., 2011). AtbZIP44 was identified as a putative regulator of the AtMAN7 expression and later validated by several molecular and physiological techniques. Co-expression in the micropylar endosperm of the putative regulator gene (AtbZIP44) and the regulated one (AtMAN7) was corroborated by microscopic techniques. A post-germination gene AtCathB3, encoding a Cathepsin B-like protease has been shown to be transcriptionally represed by the basic leucine zipper protein GBF1.


   

 

 

Immunolocalization of mannans in the seeds of Brachypodium distachyon reveals the presence of these polysacharides in the root embryo and in the coleorhiza in the early stages of germination (12 h of imbibition; hoi); mannans decrease drastically thereafter (27 hoi). Concurrently, the activity of endo-beta-mannanases increases as germination progresses. From the MAN gene family (six members) in Brachypodium, the transcripts of three of them (BdMAN2, 4 and 6) accumulate in the germinating embryos and their expression is detected in the coleorhiza and in the root, by mRNA-in situ hybridization techniques before root protrusion. These data indicate that these three genes are important for germination sensu stricto and that the coleorhiza in monocots and the micropylar endosperm in dicots may have similar functions. The BdGAMYB protein from Brachypodium distachyon interacting with BdDOF24 regulates transcription of the BdCathB, gene enconding a Cathepsin-like proteinase, during reserve mobilization in post-germination.


 

 

Besides our own research, we have had external collaborations with Spanish scientific groups from IBMCP (UPV-CSIC; Valencia), CEBAS (CSIC; Murcia), Department of Plant Physiology (USC; Santiago de Compostela), Department of Biochemistry and Molecular Biology (UC, Córdoba). Most important , through our participation in the TRILATERAL project (Transcriptional networks and their evolution in the Brassicaceae; MICINN-EUI2008-03716), Germany-France-Spain Action, we have collaborated  with important European groups, such as those of George Coupland (Max Planck Institute, MPI-Köln, Germany), Detlef Weigel (MPI-Tubingen, Germany), Vincent Colot (CNRS-INSERM, France), Haidi Quednesville (INRA-Versalles, France), Pilar Carbonero (CBGP-UPM, Spain) and Javier Paz-Ares (Centro Nacional de Biotecnología, CNB-CSIC, Spain).

 


Representative Publications

Carrillo, L., Baroja-Fernández, E., Renau-Morata, B., Muñoz, F.J., Canales, J., Ciordia, S., Yang, L., Sánchez-López, Á.M., Nebauer, S.G., Ceballos, M.G., Vicente-Carbajosa, J., Molina, R.V., Pozueta-Romero, J., Medina, J. 2023. Ectopic expression of the AtCDF1 transcription factor in potato enhances tuber starch and amino acid contents and yield under open field conditions. Frontiers in Plant Science 14. DOI: 10.3389/fpls.2023.1010669

Iglesias-Fernández, R., Vicente-Carbajosa, J. 2022. A View into Seed Autophagy: From Development to Environmental Responses. Plants 11, 3247. DOI: 10.3390/plants11233247

Pérez-Alonso, M.-M., Guerrero-Galán, C., González Ortega-Villaizán, A., Ortiz-García, P., Scholz, S.S., Ramos, P., Sakakibara, H., Kiba, T., Ludwig-Müller, J., Krapp, A., Oelmüller, R., Vicente-Carbajosa, J., Pollmann, S. 2022. The calcium sensor CBL7 is required for Serendipita indica-induced growth stimulation in Arabidopsis thaliana, controlling defense against the endophyte and K+ homoeostasis in the symbiosis. Plant, Cell & Environment. DOI: 10.1111/pce.14420

Domínguez-Figueroa, J., Carrillo, L., Renau-Morata, B., Yang, L., Molina, R.-V., Marino, D., Canales, J., Weih, M., Vicente-Carbajosa, J., Nebauer, S.G., Medina, J. 2020. The Arabidopsis Transcription Factor CDF3 Is Involved in Nitrogen Responses and Improves Nitrogen Use Efficiency in Tomato. Frontiers in Plant Science 11, 1825. DOI: 10.3389/fpls.2020.601558

Renau-Morata, B., Carrillo, L., Cebolla-Cornejo, J., Molina, R.V., Martí, R., Domínguez-Figueroa, J., Vicente-Carbajosa, J., Medina, J., Nebauer, S.G. 2020. The targeted overexpression of SlCDF4 in the fruit enhances tomato size and yield involving gibberellin signalling. Scientific Reports 10, 10645. DOI: 10.1038/s41598-020-67537-x

Katsuya-Gaviria, K., Caro, E., Carrillo-Barral, N., Iglesias-Fernández, R. 2020. Reactive Oxygen Species (ROS) and Nucleic Acid Modifications During Seed Dormancy. Plants 9, 679. DOI: 10.3390/plants9060679

Renau-Morata, B., Carrillo, L., Dominguez-Figueroa, J., Vicente-Carbajosa, J., Molina, R.V., G. Nebauer, S., Medina, J. 2020. CDF transcription factors: plant regulators to deal with extreme environmental conditions. Journal of Experimental Botany. DOI: 10.1093/jxb/eraa088

Iglesias-Fernández, R., Pastor-Mora, E., Vicente-Carbajosa, J., Carbonero, P. 2020. A Possible Role of the Aleurone Expressed Gene HvMAN1 in the Hydrolysis of the Cell Wall Mannans of the Starchy Endosperm in Germinating Hordeum vulgare L. Seeds. Frontiers in Plant Science 10, 1706. DOI: 10.3389/fpls.2019.01706

Pérez-Alonso, M.-M., Guerrero-Galán, C., Scholz, S.S., Kiba, T., Sakakibara, H., Ludwig-Müller, J., Krapp, A., Oelmüller, R., Vicente-Carbajosa, J., Pollmann, S. 2020. Harnessing symbiotic plant–fungus interactions to unleash hidden forces from extreme plant ecosystems. Journal of Experimental Botany. DOI: 10.1093/jxb/eraa040

Alvarez, J.M., Moyano, T.C., Zhang, T., Gras, D.E., Herrera, F.J., Araus, V., O’Brien, J.A., Carrillo, L., Medina, J., Vicente-Carbajosa, J., Jiang, J., Gutiérrez, R.A. 2019. Local Changes in Chromatin Accessibility and Transcriptional Networks Underlying the Nitrate Response in Arabidopsis Roots. Molecular Plant. DOI: 10.1016/j.molp.2019.09.002

Arraño-Salinas, P; Domínguez-Figueroa, J; Herrera-Vásquez, A; Zavala, D; Medina, J; Vicente-Carbajosa, J; Meneses, C; Canessa, P; Moreno, AA; Blanco-Herrera, F. 2018. "WRKY7, -11 and -17 transcription factors are modulators of the bZIP28 branch of the unfolded protein response during PAMP-triggered immunity in Arabidopsis thaliana". Plant Science. DOI: 10.1016/j.plantsci.2018.09.019".

Tenorio-Berrío, R; Pérez-Alonso, M-M; Vicente-Carbajosa, J; Martín-Torres, L; Dreyer, I; Pollmann, S. 2018. "Identification of two auxin-regulated potassium transporters involved in seed maturation". International Journal of Molecular Sciences. DOI: 10.3390/ijms19072132".

Perez-Alonso, MM; Carrasco-Loba, V; Medina, J; Vicente-Carbajosa, J; Pollmann, S. 2018. "When transcriptomics and metabolomics work hand in hand: A case study characterizing plant CDF transcription factors". High Throughput. DOI: 10.3390/ht7010007".

Carrillo-Barral, N; Matilla, AJ; Rodríguez-Gacio, MdC; Iglesias-Fernández, R. 2017. "Mannans and endo-β-mannanase transcripts are located in different seed compartments during Brassicaceae germination". Planta. DOI: 10.1007/s00425-017-2815-4".

Renau-Morata, B; Molina, RV; Carrillo, L; Cebolla-Cornejo, J; Sánchez-Perales, M; Pollmann, S; Dominguez-Figueroa, J; Corrales, AR; Flexas, J; Vicente-Carbajosa, J; MEDINA, J; Nebauer, S. 2017. "Ectopic expression of CDF3 genes in tomato enhances biomass production and yield under salinity stress conditions". Frontiers in Plant Science. DOI: 10.3389/fpls.2017.00660".

Restovic, F; Espinoza-Corral, R; Gómez, I; Vicente-Carbajosa, J; Jordana, X. 2017. "An active mitochondrial complex II present in mature seeds contains an embryo-specific iron–sulfur subunit regulated by ABA and bZIP53 and is involved in germination and seedling establishment". Frontiers in Plant Science. DOI: 10.3389/fpls.2017.00277".

Corrales, A-R; Carrillo, L; Lasierra, P; Nebauer, SG; Dominguez-Figueroa, J; Renau-Morata, B; Pollmann, S; Granell, A; Molina, R-V; Vicente-Carbajosa, J; Medina, J. 2017. "Multifaceted role of Cycling Dof Factor 3 (CDF3) in the regulation of flowering time and abiotic stress responses in Arabidopsis". Plant, Cell & Environment. DOI: 10.1111/pce.12894".

Iglesias‐Fernández, R; Matilla, A. 2016. "Flooding stress and O2-shortage in plants: An overview", p. 711-731. In P. Ahmad (ed.), Water Stress and Crop Plants: A Sustainable Approach, vol. 2. Wiley-Blackwell. DOI:

Carbonero, P; Iglesias-Fernández, R; Vicente-Carbajosa, J. 2016. "The AFL subfamily of B3 transcription factors: evolution and function in angiosperm seeds". Journal of Experimental Botany. DOI: 10.1093/jxb/erw458".

Hossain, A; Henríquez-Valencia, C; Gómez-Páez, M; Medina, J; Orellana, A; Vicente-Carbajosa, J; Zouhar, J. 2016. "Identification of novel components of the Unfolded Protein Response in Arabidopsis". Frontiers in Plant Science. DOI: 10.3389/fpls.2016.00650".

Abraham, Z; Iglesias Fernández, R; Martinez, M; Diaz, I; Carbonero, P; Vicente-Carbajosa, J. 2016. "A developmental switch of gene expression in the barley seed mediated by HvVP1 (Viviparous1) and HvGAMYB interactions". Plant Physiology. DOI: 10.1104/pp.16.00092".

Mair, A; Pedrotti, L; Wurzinger, B; Anrather, D; Simeunovic, A; Weiste, C; Valerio, C; Dietrich, K; Kirchler, T; Nägele, T; Vicente Carbajosa, J; Hanson, J; Baena-González, E; Chaban, C; Weckwerth, W; Dröge-Laser, W; Teige, M. 2015. "SnRK1-triggered switch of bZIP63 dimerization mediates the low-energy response in plants". eLIFE. DOI: 10.7554/eLife.05828".

Hartmann, L; Pedrotti, L; Weiste, C; Fekete, A; Schierstaedt, J; Göttler, J; Kempa, S; Krischke, M; Dietrich, K; Mueller, MJ; Vicente-Carbajosa, J; Hanson, J; Dröge-Laser, W. 2015. "Crosstalk between two bZIP signaling pathways orchestrates salt-induced metabolic reprogramming in Arabidopsis roots". Plant Cell. DOI: 10.1105/tpc.15.00163".

Vasquez-Soto, B; Manriquez, N; Cruz-Amaya, M; Zouhar, J; Raikhel, NV; Norambuena, L. 2015. "Sortin2 enhances endocytic trafficking towards the vacuole in Saccharomyces cerevisiae". Biological Research. DOI: 10.1186/s40659-015-0032-9".

Corrales, A-R; Nebauer, SG; Carrillo, L; Fernández-Nohales, P; Marqués, J; Renau-Morata, B; Granell, A; Pollmann, S; Vicente-Carbajosa, J; Molina, R-V; Medina, J. 2014. "Characterization of tomato Cycling Dof Factors reveals conserved and new functions in the control of flowering time and abiotic stress responses". Journal of Experimental Botany. DOI: 10.1093/jxb/ert451".

Zanin, M; Medina Alcazar, J; Vicente Carbajosa, J; Gomez Paez, M; Papo, D; Sousa, P; Menasalvas, E; Boccaletti, S. 2014. "Parenclitic networks: uncovering new functions in biological data". Scientific Reports. DOI: 10.1038/srep05112".

Herrera-Vásquez, A; Carvallo, L; Blanco, F; Tobar, M; Villarroel-Candia, E; Vicente-Carbajosa, J; Salinas, P; Holuigue, L. 2015. "Transcriptional control of glutaredoxin GRXC9 expression by a salicylic acid-dependent and NPR1-independent pathway in Arabidopsis". Plant Molecular Biology Reporter. DOI: 10.1007/s11105-014-0782-5".

Zouhar, J; Sauer, M. 2014. "Helping hands for budding prospects: ENTH/ANTH/VHS accessory proteins in endocytosis, vacuolar transport, and secretion". Plant Cell. DOI: 10.1105/tpc.114.131680".

Hentrich, M; Sánchez-Parra, B; Pérez Alonso, M-M; Carrasco Loba, V; Carrillo, L; Vicente-Carbajosa, J; Medina, J; Pollmann, S. 2013. "YUCCA8 and YUCCA9 overexpression reveals a link between auxin signaling and lignification through the induction of ethylene biosynthesis". Plant Signaling & Behavior. DOI: 10.4161/psb.26363".