Group leader: Mark Wilkinson - Investigador senior programa Isaac Peral

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Metagenomics ............ RNA Biology

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There are few tools that allow longitudinal analysis of metagenomic data subjected to distinct perturbations. We are examining longitudinal metagenomics data modelled as a Markov Decision Process (MDP). Given an external perturbation, the MDP predicts the next microbiome state in a temporal sequence, selected from a finite set of possible microbiome states. This results in a set of behaviour policies. For example, that moving from a state associated with disease to a state associated with health, requires applying or avoiding certain perturbations (interventions, food, drugs, etc.). We have shown the flexibility of this approach by applying MDPs to human gut and chick gut microbiomes, and human vaginal health, using sexual practices, nutritional intakes, probiotic treatments, and other perturbations to create the models. This novel analytical approach has applications in, for example, medicine where the MDP could suggest the sequence of perturbations (e.g. clinical interventions) to apply to follow the best path from any given starting state, to a desired (healthy) state, avoiding strongly negative states.

RNA Biology

Polyadenylation in Plant Pathogens:
We investigate the protein structure and components of the polyadenylation machinery in animals, plants, fungi and oomycetes. The specific objectives are: Using bioinformatics approaches, we will undertake:

    * Identification of different protein complexes involved in 3' end pre-mRNA processing in selected eukaryotic species with diverse lifestyles and environmental niches to gain insights into protein structure and evolution of the polyadenylation machinery in eukaryotes.
    * A survey for canonical and alternative polyadenylation signals using bioinformatic methods6,7 and available EST sequences from selected organisms. Transcriptome experiments will also be used to complete and confirm results.

Understanding mechanisms that regulate 3'UTR lengths by APA constitutes an uncovered area of research particularly in fungal pathogens of plants and animals, and generally in filamentous fungi. The cis elements present in the 3’UTRs such as microRNA target sites modulate gene expression by affecting cytoplasmic polyadenylation, subcellular localization, stability, translation and/or decay of the mRNA. Therefore, the selection of a proper 3' end cleavage site represents an important step of regulation of gene expression. We expect to gain knowledge about the involvement of APA mechanisms in the expression of genes that help to adapt organisms to specific environmental conditions.


Evolution of RNA Processing Machinery in Plant Pathogens:
Maturation of eukaryotic mRNA involves highly orchestrated cellular events that initiate with pre-mRNA formation in the nucleus by the RNA polymerase II, 5’end capping, splicing, and 3’end polyadenylation. These processes are occurring while RNA is transcribing, thus leading to a cotranscriptional mRNA processing. mRNA 3’end formation is a two-step process essential for eukaryotic gene expression. Multiple levels of regulation tightly control and coordinate these gene expression processes.
Studies on yeast and filamentous fungi have revealed that some of the cellular processes that regulate gene expression in animals and plants are not present or simply have evolved differently in these species. Previous work has shown that the RNA-binding protein Rbp35 of the rice blast fungus Magnaporthe oryzae is not present in yeasts or mammalian cells. Rbp35 is a subunit of the fungal Cleavage Factor I complex, which is part of the polyadenylation machinery. It has been shown that Rbp35 regulates the alternative polyadenylation of a subset of transcripts associated with fungal pathogenicity. These observations prompted our investigation on understanding the protein composition of the eukaryotic mRNA machineries within the different phyla of the fungal kingdom to understand their evolution and potential links to their wide range of lifestyles. For this purpose, we performed an in silico analysis and identified the core components of the RNA-associated processing machineries in fungi.





Representative Publications

García-Jiménez, B; de la Rosa, T; Wilkinson, MD. 2018. "MDPbiome: microbiome engineering through prescriptive perturbations". Bioinformatics. DOI: 10.1093/bioinformatics/bty562".

Rodríguez-Romero, J; Marconi, M; Ortega-Campayo, V; Demuez, M; Wilkinson, MD; Sesma, A. "Virulence- and signaling-associated genes display a preference for long 3′UTRs during rice infection and metabolic stress in the rice blast fungus". New Phytologist. DOI: 10.1111/nph.15405".

Wilkinson, MD; Sansone, S-A; Schultes, E; Doorn, P; Bonino da Silva Santos, LO; Dumontier, M. 2018. "A design framework and exemplar metrics for FAIRness". Scientific Data. DOI: 10.1038/sdata.2018.118".

Townend, GS; Ehrhart, F; Kranen, HJ; Wilkinson, M; Jacobsen, A; Roos, M; Willighagen, EL; Enckevort, D; Evelo, CT; Curfs, LMG. "MECP2 variation in Rett syndrome ‐ an overview of current coverage of genetic and phenotype data within existing databases". Human Mutation. DOI: 10.1002/humu.23542".

Roos, M; López Martin, E; Wilkinson, MD. 2017. "Preparing Data at the Source to Foster Interoperability across Rare Disease Resources", p. 165-179. In M. Posada de la Paz, D. Taruscio, and S. C. Groft (eds.), Rare Diseases Epidemiology: Update and Overview. Springer International Publishing, Cham. DOI: 10.1007/978-3-319-67144-4_9".

Illana, A; Marconi, M; Rodríguez-Romero, J; Xu, P; Dalmay, T; Wilkinson, MD; Ayllón, MÁ; Sesma, A. 2017. "Molecular characterization of a novel ssRNA ourmia-like virus from the rice blast fungus Magnaporthe oryzae". Archives of Virology. DOI: 10.1007/s00705-016-3144-9".

Wilkinson, MD; Verborgh, R; Bonino da Silva Santos, LO; Clark, T; Swertz, MA; Kelpin, FDL; Gray, AJG; Schultes, EA; van Mulligen, EM; Ciccarese, P; Kuzniar, A; Gavai, A; Thompson, M; Kaliyaperumal, R; Bolleman, JT; Dumontier, M. 2017. "Interoperability and FAIRness through a novel combination of Web technologies". PeerJ Computer Science. DOI: 10.7717/peerj-cs.110".

Mons, B; Neylon, C; Velterop, J; Dumontier, M; da Silva Santos, LOB; Wilkinson, MD. 2017. "Cloudy, increasingly FAIR; revisiting the FAIR Data guiding principles for the European Open Science Cloud". Information Services & Use. DOI: 10.3233/isu-170824".

Rodríguez Iglesias, A; Rodríguez González, A; Irvine, AG; Sesma, A; Urban, M; Hammond-Kosack, KE; Wilkinson, MD. 2016. "Publishing FAIR Data: an exemplar methodology utilizing PHI-base". Frontiers in Plant Science. DOI: 10.3389/fpls.2016.00641".

Wilkinson, MD; Dumontier, M; Aalbersberg, IJ; Appleton, G; Axton, M; Baak, A; Blomberg, N; Boiten, J-W; da Silva Santos, LB; Bourne, PE; Bouwman, J; Brookes, AJ; Clark, T; Crosas, M; Dillo, I; Dumon, O; Edmunds, S; Evelo, CT; Finkers, R; Gonzalez-Beltran, A; Gray, AJG; Groth, P; Goble, C; Grethe, JS; Heringa, J; ’t Hoen, PAC; Hooft, R; Kuhn, T; Kok, R; Kok, J; Lusher, SJ; Martone, ME; Mons, A; Packer, AL; Persson, B; Rocca-Serra, P; Roos, M; van Schaik, R; Sansone, S-A; Schultes, E; Sengstag, T; Slater, T; Strawn, G; Swertz, MA; Thompson, M; van der Lei, J; van Mulligen, E; Velterop, J; Waagmeester, A; Wittenburg, P; Wolstencroft, K; Zhao, J; Mons, B. 2016. "The FAIR Guiding Principles for scientific data management and stewardship". Scientific Data. DOI: 10.1038/sdata.2016.18".

Aranguren, ME; Wilkinson, MD. 2015. "Enhanced reproducibility of SADI web service workflows with Galaxy and Docker". GigaScience. DOI: 10.1186/s13742-015-0092-3".

Nakada, T; Boyd, JH; Russell, JA; Aguirre-Hernández, R; Wilkinson, MD; Thair, SA; Nakada, E; McConechy, MK; Fjell, CD; Walley, KR. 2015. "VPS13D gene variant is associated with altered IL-6 production and mortality in septic shock". Journal of Innate Immunity. DOI: 10.1159/000381265".

Pawluczyk, M; Weiss, J; Links, MG; Egaña Aranguren, M; Wilkinson, MD; Egea-Cortines, M. 2015. "Quantitative evaluation of bias in PCR amplification and next-generation sequencing derived from metabarcoding samples". Analytical and Bioanalytical Chemistry. DOI: 10.1007/s00216-014-8435-y".

Marconi, M; Rodriguez-Romero, J; Sesma, A; Wilkinson, MD. 2014. "Bioinformatics tools for Next-Generation RNA sequencing analysis ", p. 371-391. In A. Sesma and T. von der Haar (eds.), Fungal RNA Biology. Springer International Publishing Switzerland. DOI: 10.1007/978-3-319-05687-6_15".

Katayama T; Wilkinson M; Aoki-Kinoshita K; Kawashima S; Yamamoto Y; Yamaguchi A; Okamoto S; Kawano S; Kim J-D; Wang Y; Wu H; Kano Y; Ono H; Bono H; Kocbek S; Aerts J; Akune Y; Antezana E; Arakawa K; Aranda B; Baran J; Bolleman J; Bonnal R; Buttigieg P; Campbell M; Chen Y; Chiba H; Cock P; Cohen K; Constantin A. 2014. "BioHackathon series in 2011 and 2012: penetration of ontology and linked data in life science domains". J. Biomed. Semantics 5:5.

Dumontier, M; Baker, C; Baran, J; Callahan, A; Chepelev, L; Cruz-Toledo, J; Del Rio, N; Duck, G; Furlong, L; Keath, N; Klassen, D; McCusker, J; Queralt-Rosinach, N; Samwald, M; Villanueva-Rosales, N; Wilkinson, M; Hoehndorf, R. 2014. "The Semanticscience Integrated Ontology (SIO) for biomedical research and knowledge discovery". Journal of Biomedical Semantics 5:14.

Samadian S; McManus B; Wilkinson M. 2014. "Automatic detection and resolution of measurement-unit conflicts in aggregated data". BMC Med. Genomics 7:S12.

Egana Aranguren, M; Rodriguez Gonzalez, A; Wilkinson, MD. 2014. "Executing SADI services in Galaxy". Journal of Biomedical Semantics. DOI: 10.1186/2041-1480-5-42".

Luciano, JS; Cumming, GP; Kahana, E; Wilkinson, MD; Brooks, EH; Jarman, H; McGuinness, DL; Levine, MS. 2014. "Health Web Science". Foundations and Trends® in Web Science. DOI: 10.1561/1800000019".

Rodríguez González, A; Callahan, A; Cruz-Toledo, J; Garcia, A; Egaña Aranguren, M; Dumontier, M; Wilkinson, M. 2014. "Automatically exposing OpenLifeData via SADI semantic Web Services". Journal of Biomedical Semantics. DOI: 10.1186/2041-1480-5-46

Egaña Aranguren, M; Fernández-Breis, JT; Antezana, E; Mungall, C; Rodríguez González, A; Wilkinson, MD. 2013. "OPPL-Galaxy, a Galaxy tool for enhancing ontology exploitation as part of bioinformatics workflows". Journal of Biomedical Semantics. DOI: 2041-1480-4-2 [pii] 10.1186/2041-1480-4-2".

Katayama, T; Wilkinson, MD; Micklem, G; Kawashima, S; Yamaguchi, A; Nakao, M; Yamamoto, Y; Okamoto, S; Oouchida, K; Chun, HW; Aerts, J; Afzal, H; Antezana, E; Arakawa, K; Aranda, B; Belleau, F; Bolleman, J; Bonnal, RJ; Chapman, B; Cock, PJ; Eriksson, T; Gordon, PM; Goto, N; Hayashi, K; Horn, H; Ishiwata, R; Kaminuma, E; Kasprzyk, A; Kawaji, H; Kido, N; Kim, YJ; Kinjo, AR; Konishi, F; Kwon, KH; Labarga, A; Lamprecht, AL; Lin, Y; Lindenbaum, P; McCarthy, L; Morita, H; Murakami, K; Nagao, K; Nishida, K; Nishimura, K; Nishizawa, T; Ogishima, S; Ono, K; Oshita, K; Park, KJ; Prins, P; Saito, TL; Samwald, M; Satagopam, VP; Shigemoto, Y; Smith, R; Splendiani, A; Sugawara, H; Taylor, J; Vos, RA; Withers, D; Yamasaki, C; Zmasek, CM; Kawamoto, S; Okubo, K; Asai, K; Takagi, T. 2013. "The 3rd DBCLS BioHackathon: improving life science data integration with Semantic Web technologies". Journal of Biomedical Semantics. DOI: 2041-1480-4-6 [pii] 10.1186/2041-1480-4-6".

Luciano, JS; Cumming, GP; Wilkinson, MD; Kahana, E. 2013. "The emergent discipline of health web science". Journal of Medical Internet Research. DOI: v15i8e166 [pii] 10.2196/jmir.2499".

McCarthy, L; Vandervalk, B; Wilkinson, M. 2012. "SPARQL Assist language-neutral query composer" BMC bioinformatics, vol. 13 Suppl 1, no. Suppl 1, p. S2.

Samadian, S; McManus, B; Wilkinson, M.D. 2012. "Extending and encoding existing biological terminologies and datasets for use in the reasoned semantic web" Journal of biomedical semantics, vol. 3, no. 1, p. 6, Jul.

Rodríguez-González, A; Torres-Niño, J; Mayer, M. A; Alor-Hernandez, G; Wilkinson, M.D. 2012. "Analysis of a multilevel diagnosis decision support system and its implications: a case study" Computational and Mathematical Methods in Medicine, vol. 2012, pp. 1-9.

Wood, I; Vandervalk, B; McCarthy, L; Wilkinson, M. 2012. "OWL-DL Domain-Models as Abstract Workflows" in Leveraging Applications of Formal Methods, Verification and Validation. Applications and Case Studies, T. Margaria and B. Steffen, Eds. Berlin/Heidelberg: Springer, pp. 56-66.

Centro de Biotecnología y Genómica de Plantas UPM – INIA Parque Científico y Tecnológico de la U.P.M. Campus de Montegancedo
Autopista M-40, Km 38 - 28223 Pozuelo de Alarcón (Madrid) Tel.: +34 91 4524900 ext. 1806 / +34 91 3364539 Fax: +34 91 7157721. Localización y Contacto

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