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Estudio de las redes reguladoras implicadas en la respuesta a disponibilidad de nitrato en Arabidopsis

Plants are sessile organisms that cannot relocate to find their optimal living conditions. In order to cope with a changing environment, plants rapidly adjust gene expression, metabolism, and physiology to optimize their growth, development, and reproduction. Nitrogen (N) nutrient availability is a strong environmental factor that affects many plant processes. Nitrate (NO3−) is the main N source for many plants and acts as a signal that regulates global gene expression, metabolism, physiology, and growth and development. In Arabidopsis thaliana, NO3− induces a rapid, broad-range modulation of gene expression that affects a myriad of genes.

The present study, is
collaborative work between researchers of different universities, including Berkeley (USA), Wisconsin-Madison (USA), Michigan State (USA), Yangzhou (China), Pontifical Universidad Católica (Chile), Politécnica of Madrid and INIA (Joaquín Medina). This cooperative effort has allowed a multidisciplinary global analysis of the changes in gene expression in response to nitrate in plants. In this work, data obtained through different omic techniques were integrated to study the relationship between accessibility of specific regions of the genome associated with nitrate-regulated genes and specific regulatory factors.

The results obtained indicate that nitrate produces very rapid and dynamic changes in gene expression that are intimately related to the structure of chromatin. In addition, these analyses genomic regions and determining regulatory factors in the response to nitrate availability have been determined. The importance of these results is that constitute a step forward in the identification of key genes for the development of improved crop varieties which are more efficient in the use of resources such as fertilizers and therefore to enhanced their yield in sustainable agriculture.

Figure. NO3− triggers rapid change sin RNA polymerase II (RNPII) patterns. (a) Genes that show a significant increase/decrease in RNPII occupancy in response to NO3− treatments. More genes are upregulated than downregulated at RNPII occupancy level (p < 1E−13). (b) 69% Transcripts induced by NO3− show a significant increase in RNPII occupancy in response to NO3−. (c) RNPII occupancy increases in NIA1 and NIR genes in response to NO3−. (d) Transcription Factor Network Integrate Transcriptional Regulation and Connectivity Features of Genes in the NO3− Response.

Publicación Original:

Alvarez, J.M., Moyano, T.C., Zhang, T., Gras, D.E., Herrera, F.J., Araus, V., O’Brien, J.A., Carrillo, L., Medina, J., Vicente-Carbajosa, J., Jiang, J., Gutiérrez, R.A. 2019. Local Changes in Chromatin Accessibility and Transcriptional Networks Underlying the Nitrate Response in Arabidopsis Roots. Molecular Plant. DOI: 10.1016/j.molp.2019.09.002


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