Spectral confocal microscope Leica TCS-SP8

 

 

Inverted microscope Leica DMI6000CS:

Type

Excitation light

Excitation filter

Dichroic mirror

Emission filter

A4

UV

BP 360/40

400

BP 470/40

CFP

violet/blue

BP 436/20

455

BP 480/40

GFP

blue

BP 470/40

500

BP 525/50

I3

blue

BP 450-490

510

LP 515

N2.1

green

BP 515-560

580

LP 590

 

Type

Magnification/numerical aperture

Technique*

Immersion

PL APO CS

10x/0.4

BF,FLUO

 

PL APO CS

20x/0.70

BF,FLUO

 

PL APO CS2

20X/0.75

BF, DIC, FLUO

water, glycerol, oil

PL APO CS2

40x/1.1

BF, DIC, FLUO

water

PL APO CS2

63x/1.4

BF, DIC, FLUO

oil

 

*BF = bright field, DIC = differential interference contrast, FLUO = fluorescence

 

Confocal module

FOV scanner:

Lasers:

Detectors:

LIAchroic beam splitter, which includes the dichroic mirrors:

Galvanometric Z-stage:

Motorized XY scanning stage

Software

LAS X with wizard for FRET & FRAP analysis and 3D viewer

 

Galery...