Super-resolution confocal microscope Zeiss LSM 880 with fast Airyscan

 

 

 

 

Upright Axio Imager Z2 microscope
  • touch screen to control all functions

  • metal halide lamp (120 W)

  • fluorescence cubes:

Type

Excitation light

Excitation filter

Emission filter

DAPI

UV

356

BP 455/50

GFP

blue

BP 470/40

BP 525/50

DsRed

green

BP 530-585

LP 615

 

Objectives:

Type

Magnification/numerical aperture

Technique

Inmersión

Plan-Apochromat

10x/0.45

 

 

Plan-Apochromat

25x/0.80

DIC

water, glycerol, oil

Plan-Apochromat

40X/1.2

DIC

water, glycerol, oil

Plan-Apochromat

63x/1.4

DIC

oil

 

CCD camera Axiocam 503 colour
  • pixels: 1936 x 1460
  • pixel size: 4.54 μm x 4.54 μm
Confocal module

Lasers

  • 405, 488, 458, 514, 561 and 633 nm

Detectors

  • 3 spectral PMTs
  • Airyscan detector for super-resolution
  • 1 PMT for transmitted light

Motorized Z scanning stage

  • speed: 100 mm/s
  • resolution: 0,1 µm
  • reproducibility <1 µm

Motorized Z stage

  • range: 500 μm
  • speed: 5 mm/sec
  • reproducibility: +/- 5 nm

Software

  • ZEN black: image acquisition
  • ZEN blue: image processing (3D, colocalisation)

Centre for Plant Biotechnology and Genomics UPM – INIA Parque Científico y Tecnológico de la U.P.M. Campus de Montegancedo
Autopista M-40, Km 38 - 28223 Pozuelo de Alarcón (Madrid) Tel.: +34 91 0679100 ext. 79100 Fax: +34 91 7157721. Location and Contact

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