Spectral confocal microscope Leica TCS-SP8

 

 

Inverted microscope Leica DMI6000CS:

  • touchscreen STP6000

  • smart move (moves the stage in XY and Z)

  • metal halide bulb EL6000 (120 W)

  • Fluorescence clubes:

Type

Excitation light

Excitation filter

Dichroic mirror

Emission filter

A4

UV

BP 360/40

400

BP 470/40

CFP

violet/blue

BP 436/20

455

BP 480/40

GFP

blue

BP 470/40

500

BP 525/50

I3

blue

BP 450-490

510

LP 515

N2.1

green

BP 515-560

580

LP 590

 

  • objectives:

Type

Magnification/numerical aperture

Technique*

Immersion

PL APO CS

10x/0.4

BF,FLUO

 

PL APO CS

20x/0.70

BF,FLUO

 

PL APO CS2

20X/0.75

BF, DIC, FLUO

water, glycerol, oil

PL APO CS2

40x/1.1

BF, DIC, FLUO

water

PL APO CS2

63x/1.4

BF, DIC, FLUO

oil

 

*BF = bright field, DIC = differential interference contrast, FLUO = fluorescence

 

Confocal module

FOV scanner:

  • speed up to 1.800 Hz (3.600 Hz in bidirectional mode)
  • up to 7 fps @ 512 x 512 px
  • image format up to 8.192 x 8.192 px

Lasers:

  • CW (405 nm). 50 mW
  • Ar (458, 488 y 514 nm). 100 mW
  • DPSS (561 nm). 20 mW
  • He-Ne (633 nm). 10 mW

Detectors:

  • 4 spectral for fluorescence and reflection (2 PMT and 2 HyD)
  • 1 (non-confocal) for transmitted light

LIAchroic beam splitter, which includes the dichroic mirrors:

  • substrate (405)
  • 488/561/633
  • 458/514/561
  • 476/488
  • RT 15/85 (reflection)
  • 488/561

Galvanometric Z-stage:

  • min. step 20 nm
  • range 1500 µm

Motorized XY scanning stage

  • speed: 10 mm/s
  • resolution 0,02-0,04 µm
  • reproducibility <1 µm

Software

LAS X with wizard for FRET & FRAP analysis and 3D viewer

 

Galery...

 

Centre for Plant Biotechnology and Genomics UPM – INIA Parque Científico y Tecnológico de la U.P.M. Campus de Montegancedo
Autopista M-40, Km 38 - 28223 Pozuelo de Alarcón (Madrid) Tel.: +34 91 0679100 ext. 79100 Fax: +34 91 7157721. Location and Contact

Síguenos en: