Spectral confocal microscope Leica TCS-SP8

 

 

Inverted microscope Leica DMI6000CS:

  • touchscreen STP6000

  • metal halide bulb EL6000 (120 W)

  • fluorescence cubes:

 

Type

 

Excitation light

 

Excitation filter

 

Dichroic mirror

 

Emission filter

A4

 

UV

BP 360/40

400

BP 470/40

CFP

 

violet/blue

BP 436/20

455

BP 480/40

GFP

 

blue

BP 470/40

500

BP 525/50

I3

 

blue

BP 450-490

510

LP 515

N2.1

 

green

BP 515-560

580

LP 590

 

objectives:

 

Type

 

Magnification/numerical aperture

 

Technique

 

Immersion

PL APO CS

10x/0.4

 

 

PL APO CS

20x/0.70

 

 

PL APO CS2

20X/0.75

Nomarski

 

water, glycerol, oil

PL APO CS2

40x/1.1

Nomarski

 

water

PL APO CS2

63x/1.4

Nomarski

oil

 

 

Ultraspectral confocal module Leica TCS SP8

1) FOV scanner:

  • speed up to 1.800 Hz (3.600 Hz in bidirectional mode)
  • up to 7 fps @ 512 x 512 px
  • image format up to 8.192 x 8.192 px

2) Detectors:

  • 4 spectral for fluorescence and reflection (2 PMT and 2 HyD)
  • 1 (non-confocal) for transmitted light (Nomarski in objectives 40x and 63x)

3) LIAchroic beam splitter, which includes the dichroic mirrors:

  • substrate (405)
  • 488/561/633
  • 458/514/561
  • 476/488
  • RT 15/85 (reflection)
  • 488/561

4) Lasers:

  • CW (405 nm). 50 mW
  • Ar (458, 488 y 514 nm). 100 mW
  • DPSS (561 nm). 20 mW
  • He-Ne (633 nm). 10 mW

5) Galvanometric Z-stage:

  • min. step 20 nm
  • range 1500 µm

6) Motorized XY scanning stage

  • speed: 10 mm/s
  • resolution 0,02-0,04 µm
  • reproducibility <1 µm

Software

LAS X with wizard for FRET & FRAP analysis and 3D viewer

 

Galery...

Centre for Plant Biotechnology and Genomics UPM – INIA Parque Científico y Tecnológico de la U.P.M. Campus de Montegancedo
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