Microscopy

Technical Responsible: Pablo González-Melendi de León - Associate Professor

Personnel:

• González Carneros, Carlos - Technician

Service member of:

Spanish Network of Advanced Light Microscopy (REMOA)

Platform of Microscopy for Biosciences. Network of Laboratories and Infrastructures of the Community of Madrid

Leica TCS-SP8 confocal microscope

Super-resolution confocal microscope Zeiss LSM 880 with fast Airyscan

Services and techniques

• Training in the use of imaging equipment (microscopes, cameras).
• Bright field microscopy for histochemistry.
• Bioluminiscence imaging.
• Fluorescence microscopy for subcellular localizations, molecular interactions, plant-microorganisms interactions.
• Confocal microscopy for 3d reconstructions of fluorescent markers.
• Confocal microscopy with super-resolution (Airyscan, 120 nm).

Microtomes and sample preparation

• Microtome Leica RM2135
• Paraffin-wax dispensers Selecta and Leica EG 1110
• Hybridisation oven Amersham
• Vibratomo 1000 plus

Microscopes

• Upright microscope Zeiss Axiophot [Gallery]
• Inverted microscope Leica DM IRB
• Upright microscope Leica DM RB
• Stereomicroscope Leica MZ10 F
• Upright microscope Leica DM2000
• Stereomicroscope Olympus SZX95
• Stereomicroscope Leica MZ9

Confocal microscope

• Spectral confocal microscope Leica TCS-SP8 [Gallery]

• Super-resolution confocal microscope Zeiss LSM 880 with fast Airyscan

CCD camera

• CCD camera NightOwl LB 983 NC100. Berthold

Equipment for high sensitivity image acquisition (FLumaZone)

• Stereomicroscope LEICA M205FA for fluorescence and bioluminiscence
• High sensitivity Electron Multiplying Hamamatsu ImagEM X2 EM-CCD camera
• Metamorph for image acquisition, integration and analysis

Fluorescence motorized microscope with a high sensitive camera

• Inverted microscope Leica Dmi8
• Digital camera Hamamatsu ORCA Flash 4.0 LT

Click here to download the Fiji software for confocal imaging management.