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Automated design and implementation of a genetic circuit

The design and implementation of Boolean logic circuits in microorganisms is a fundamental part of synthetic biology. We use design software tools to build a NOR logic gate in the bacteria , and show it performs as expected without error-prone iteration. We highlight the benefits of optimizing design tools to each organism.

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Original Paper:

Tas, H., Grozinger, L., Goñi-Moreno, A., de Lorenzo, V. 2021. Automated Design and Implementation of a NOR Gate in Pseudomonas Putida. Synthetic Biology. DOI: 10.1093/synbio/ysab024

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