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The bacteria Pseudomonas putida presents a highly precise intracellular organisation

We analyzed the spacial structure that allows information flows between DNA, RNA and proteins. In contrast to the common simplification, that considers the intracellular space as homogeneous, we show that the tridimensional organization of the regulatory machinery determines the function of essential processes, as gene expression. This enables new avenues for synthetic biology research, in which is not only the DNA sequence that matters, but also the spatial structure that processes that information. Specifically, here we analyzed the expression of the genes involved in the TOL catabolic pathway of Pseudomonas putida mt-2.


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Original Paper:

Kim, J., Goñi-Moreno, A., Lorenzo, V. de 2021. Subcellular Architecture of the xyl Gene Expression Flow of the TOL Catabolic Plasmid of Pseudomonas putida mt-2. mBio 12. DOI: 10.1128/mBio.03685-20

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