In this work, we have used the TPs of four Arabidopsis thaliana proteins, three related to photosynthesis (chlorophyll a / b binding protein, Rubisco activase) and photoprotection (tocopherol cyclase) and one involved in the assimilation of ammonium into amino acids, and whose expression is more abundant in the root (ferredoxin-dependent glutamate synthase 2), to determine if they were capable of mediating the import of a recombinant protein in plastids of different tissues of a dicotyledonous and another monocotyledonous species.

Our results show that Arabidopsis photosynthetic protein TPs also mediate importation into plastids in callus, leaves and roots of rice, with almost 100% efficiency, providing new biotechnological tools that could be useful for the development of breeding strategies. cultures that require expression of recombinant proteins in a plastid-directed manner.