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A technique for the identification in vivo of the DNA-binding sites for proteins that regulate gene expression in plants

The transcriptional regulation of specific genes is a crucial step in the response of plants to environmental factors and developmental cues. Progress in understanding how a variety of biological processes are regulated in plants depends on enlightening the molecular mechanisms that control gene expression. In this work we describe a straightforward procedure for the identification of the DNA-binding sites of Arabidopsis proteins of interest through Chromatin Immunoprecipitation (ChIP). This method has proven useful for the characterization of transcription factors, chromatin-related proteins, and posttranslationally modified histones.


Original Paper:

Jarillo, JA; Komar, DN; Piñeiro, M. 2018. "The use of the chromatin immunoprecipitation technique for in vivo identification of plant protein–DNA interactions", p. 323-334. In L. Oñate-Sánchez (ed.), Two-Hybrid Systems: Methods and Protocols. Springer New York, New York, NY. DOI: 10.1007/978-1-4939-7871-7_23".

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