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Production of hydrogen peroxide by the plant contributes to limit a virus infection

The rapid production of reactive oxygen species (ROS), is one of the first signals observed when the plant is attacked by pathogens. These ROS are mainly produced by a family of NADPH oxidases that lead to the accumulation of H2O2 to mediate the establishment of the defenses. A recent collaborative work between Miguel Angel Torres, from the Plant Immunity and Resistance to Necrotrophic Pathogens group at the CBGP, and the group led by Professor Otulak-Koziel from SGG Warsaw, investigates the role of the NADPH oxidases in the response of the model plant Arabidopsis to Turnip mosaic virus, a Potyvirus of wide host range that causes important loses in Brassicaceae crops. The study performs immune quantification of the virus, combined with in situ detection in electron microcrographs of viral particles as well as H2O2. Comparative studies indicate that one NADPH oxidase is specifically required to limit virus accumulation whereas a different one has a role in susceptibility with a possible function in systemic signal transport. This study illustrates that different pools of ROS signals can play different/opposed roles in plant immunity.

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The Arabidopsis NAPDH oxidase mutant
rbohD displays greater virus accumulation than Col-0 wild type after inoculation with Turnip mosaic virus. Otulak-Kozieł, K., Kozieł, E., Bujarski, J.J., Frankowska-Łukawska, J., Torres, M.A. 2020. Respiratory Burst Oxidase Homologs RBOHD and RBOHF as Key Modulating Components of Response in Turnip Mosaic Virus-Arabidopsis thaliana (L.) Heyhn System. International Journal of Molecular Sciences, 21, 8510.

Original Paper:

Otulak-Kozieł, K., Kozieł, E., Bujarski, J.J., Frankowska-Łukawska, J., Torres, M.A. 2020. Respiratory Burst Oxidase Homologs RBOHD and RBOHF as Key Modulating Components of Response in Turnip Mosaic Virus-Arabidopsis thaliana (L.) Heyhn System. International Journal of Molecular Sciences 21, 8510. DOI: 10.3390/ijms21228510

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