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A modification of a plant cell death staining protocol avoids the use of a highly carcinogenic product for researchers

The classic trypan blue staining protocol used for detection and quantification of cell death in various organisms has been improved in order to avoid the use of trichloroacetaldehyde hydrate or "chloral hydrate", a highly toxic and carcinogenic product, making this method of staining safer and faster. The protocol is specifically applied to detect cell death in Arabidopsis leaves during the course of infection with the necrotrophic fungus Botrytis cinerea.



Original Paper:

Fernández-Bautista, N; Domínguez-Núñez, JA; Castellano Moreno, MM; Berrocal-Lobo, M. 2016. "Plant tissue trypan blue staining during phytopathogen infection". Bio-protocol. DOI: 10.21769/BioProtoc.2078".

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